大鼠虚热证模型的建立与评价

目的:对热性中药致虚热证模型的复制和评价进行探究。方法:使用不同剂量热性中药附子、干姜、肉桂灌胃大鼠。每天观察大鼠的活动状态、毛发光泽、爪色、二便等一般状况,每天定时测定大鼠的肛温、粪便量、尿液量、饮水量、饮食量,并于体液消耗量出现明显差异时采集血液和相关组织器官,测定血清三碘甲状腺原氨酸(T3),甲状腺素(T4),血浆环磷酸腺苷(cAMP)及环磷酸鸟苷(cGMP)含量的变化和相关脏器指数。结果:与空白组比较,各模型组大鼠饮水量、体液消耗量大多数升高,动物出现虚热证的证候;且血清中T4含量呈增高趋势,T3/T4降低。模型低剂量组和模型中剂量组的cAMP和cGMP含量与空白组比较大多数显著升高,而模型高剂量组含量则有所降低。结论:灌胃热性中药可以复制出虚热证模型,体液消耗量的增加对虚热证模型复制有一定的指导意义。

Establishment and Evaluation of Rat Model with Deficient Heat Syndrome

Objective: To study on establishing the model of deficient heat syndrome due to insufficiency of Yin fluid in rats by Chinese medicinal herbs with heat property. Method: Different doses of Aconiti Lateralis Radix Praeparata,Zingiberis Rhizoma and Cinnamomi Cortex were administered intragastrically to rats.Rats'' ordinary situation was observed,included active state,hair luster,claw color,urine and stool.Rectal temperature,food intake,water intake,urine output and fecal amount were recorded at the same time in every day,the changes of triiodothyronine(T3) and thyroxine(T4) in serum,cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP) in plasma and indexes of relevant organs were detected when body fluid consumption appeared obvious difference. Result: The water intake and body fluid consumption of model groups were mostly increased by comparing with those of the control group,rats showed manifestations of heat syndrome due to insufficiency of Yin fluid.T4 in serum showed a increasing trend,but T3/T4 levels showed the trend of decline.Compared with the control group,most of cAMP and cGMP in the low and middle dose of model groups increased significantly,while the high dose of model group decreased. Conclusion: Deficient heat syndrome model can be established by administering Chinese medicinal herbs with heat property,increasing of body fluid consumption has some guiding significance for replication of deficient heat syndrome model.