川芎嗪对哮喘大鼠Th2型细胞因子作用的研究

目的 探讨川芎嗪对哮喘大鼠白细胞介素-4（IL-4）、白细胞介素-13（IL-13）水平的影响及其防治哮喘的作用机制。方法 60只SD大鼠随机分为正常对照组、哮喘模型组、地塞米松组、小剂量及大剂量川芎嗪组、联合用药组，以卵蛋白致敏和激发制备大鼠哮喘模型，用酶联免疫吸附试验（ELISA）检测支气管肺泡灌洗液（BALF）中IL4、IL—13的水平。结果 哮喘模型组BALF中IL-4、IL—13水平均高于正常对照组（P〈0．001）；地塞米松组和川芎嗪治疗各组均显著低于哮喘模型组（P〈0．001）；川芎嗪大剂量组和联合用药组降低IL-4和IL—13的作用与地塞米松组无显著性差异（P〉0．05）；川芎嗪大剂量组降低IL-4的作用与川芎嗪小剂量组无显著性差异（P〉0．05），川芎嗪大剂量组降低IL—13的作用优于川芎嗪小剂量组（P〈0．05）；BALF中IL-4和TL—13水平呈显著正相关（r=0．714，P〈0．01）。结论 川芎嗪通过抑制IL-4和IL—13的水平而抑制哮喘气道炎症。

Effects of ligustrazine on the levels of IL-4,IL-13 in asthmatic rats

Objective To explore the changes of the levels of interleukin-4(IL-4),interleukin-13(IL-13) in asthmatic rats and to evaluate the influence of ligustrazine on them.Methods Sixty male SD rats were randomly divided into 6 experimental groups(10 in each): normal control group,asthmatic model group,desamethasone group,low-dose of ligustrazine group,high-dose of ligustrazine group,combinative therapy group.The asthmatic model was established by repeated inhalation of ovalbulium.The levels of IL-4 and IL-13 in broncho-alveolar fluid(BALF) were measured by ELISA.Results The IL-4 and IL-13 levels were much higher(P<0.001) in BALF in asthmatic model group than those in normal control group.The IL-4 and IL-13 levels in each treatment group were significantly lower than those in asthmatic model group(P<0.05),but were higher than those in normal control group(P<0.05),but the levels in high-dose of ligustrazine group and combinative therapy group had no significant difference with those in desamethasone group(P>0.05).The IL-4 level in BALF in high-dose of ligustrazine group had no significant difference with that in low-dose of ligustrazine group((P>)0.05).The IL-13 level in BALF in high-dose of ligustrazine group was lower than that in low-dose of ligustrazine group(P<0.05).A positive correlation between IL-4_()and IL-13 levels in BALF was demonstrated(r=0.714,(P<)0.01).Conclusion ligustrazine can downregulate the levels of IL-4 and IL-13 in asthmatic rats and inhibit the airway inflammation.