原苏木素A对心脏移植后大鼠T淋巴细胞亚群的影响

目的探讨苏木醇提单体原苏木素A对心脏移植后大鼠T淋巴细胞亚群的免疫抑制和对心肌的保护作用。方法应用大鼠腹腔同种异位心脏移植模型。实验分为原苏木素A组、环孢素组、对照组A（手术不给药组）、对照组B（不给药不手术组）。术前2天给药，术后第7天采血，应用流式细胞仪检测外周血T淋巴细胞亚群的变化，并检测心肌组织的病理改变。结朵与心脏移植对照组比较，原苏木素A组、环孢素A组术后3天和7天时均减轻心脏移植后急性排斥反应所引起的心肌病理损害（P〈0．01）；与对照组B相比较，对照组ACD4^＋／CD8^＋明显升高（P〈0．01），环孢索A组、原苏木素A组则可抑制CD4^＋／CD8^＋的升高。结论苏木醇提单体原苏木素A对心脏移植后发生的急性免疫排斥反应有明显的抑制作用，对心肌有明显的保护作用。

Effect of protosappanin A on T lymphocyte subsets after rat heart transplantation

Objective To discuss the effect of protosappanin A(one of ethanol extract of Caesalpinia Sappan L)on the change of T lymphocyte subsets and its protective effect on myocardial tissues after allograft rat heart transplantation.Methods The cardic allograft rats were randomly divided into 4 groups:protosappanin A group,Cyclosporin A group,control group A(heart transplant group)and control group B(no treatment).Animals were given drugs 2 days before operation.The changes of T lymphocyte subsets were measured 7 days after transplantation and myocardial pathology grade were observed.Results The pathological grade in protosappanin A group and Cyclosporin A group were significantly lower than that in control group A on the 3rd and the 7th postoperative day(P<0.01).In control group A,the ratio of CD4~+/CD8~+was obviously increased compared with control group B,while protosappanin A and Cyclosporin A cound inhibit the elevation of blood CD4~+/CD8~+on the 7th day after heart transplantation.Conclusion Protosappanin A shows certain immunosuppressive effect on acute rejection after heart transplantation and protective function for myocardial tissues.