糖尿病大鼠肾脏RAGE的表达与足细胞损伤的相关性研究

目的：探讨大鼠肾组织中糖基化终产物受体的表达和肾小球足细胞的相关性及其在糖尿病肾病中的临床意义。方法：用免疫组化方法检测链脲佐菌素(STZ)诱发糖尿病及正常对照Wistar大鼠肾脏中糖基化终产物受体、结蛋白的表达，采用图像分析软件定量计数肾小球足细胞数。对大鼠肾小球糖基化终产物受体的表达与肾小球足细胞进行相关分析。 结果：与对照组相比，糖尿病组大鼠肾小球糖基化终产物受体、结蛋白表达明显增加（P<0.01），肾小球足细胞明显减少（P<0.01）；大鼠肾小球糖基化终产物受体的表达与肾小球足细胞呈负相关(r=-0.736，P<0.01)。结论：RAGE介导的细胞信号途径可能参与了糖尿病时足细胞病变的发生。

Exression of RAGE in diabetic rat kidney tissue: relationbetween RAGE and podocyte injury

Objective: To evaluate the changes of RAGE(receptor for advanced glycation endproducts) expression and podocytes in renal tissues of diabetic and control rats,and explore the relation between RAGE and podocyte injuries.Methods: Adult male Wistar rats were injected intravenously with streptozotocin(STZ) to induce diabetes.The rats were sacrificed eight weeks after the induction of diabetes.The renal RAGE and desmin expressions were accessed by semi-quantitative immunohistochemistry.The average podocyte number of each glumerulus was determined by dividing the total podocytes by the glomerular profile area as determined by point counting.And correlation analysis was used to evaluate the relationship between RAGE expression and podocytes of each glomerulus.Results: Compare with control rats,the expressions of RAGE and desmin were increased remarkably(P<0.01),and the podocytes of each glomerulus was decreased significantly(P<0.01) in diabetic rats.There was a negtive relationship between the RAGE expression and the podocytes of each glomerulus(r=-0.736,P<0.01).Conclusion: RAGE may play an important role in the progression of diabetic nephropathy.