荧光原位杂交研究乙型肝炎病毒在孕妇外周血淋巴细胞染色体上的整合

目的 采用荧光原位杂交(FISH)技术检测乙型肝炎病毒(HBV)携带孕妇外周血淋巴细胞染色体，明确是否存在ltBV整合。方法选择我院分娩者中136例ltBV携带孕妇及28名健康对照者，制备其外周血淋巴细胞染色体标本，以地高辛标记的}tBV探针进行原位杂交。结果136例中，有4例患者的外周血淋巴细胞分裂期染色体上可观察到绿色荧光信号，占2．94％，均为双阳组孕妇，HBV呈低频率整合(1．00％～1．67％)，整合位点呈随机分布。84例单阳组HBsAg( )]孕妇及28名健康对照标本中均未检出阳性信号。结论 应用FISH技术可检测外周血淋巴细胞染色体上整合型HBV。HBV携带孕妇的外周血淋巴细胞染色体上存在整合型HBV，使妊娠期HBV感染的危害不仅为母婴问的垂直传播，而且可能发生基因遗传，成为一种遗传性传染性疾病和肝癌高发的重要危险因素。

Detection of HBV virus integration in PBMC chromosome of pregnant women using fluorescence in-situ hybridization

Objective Using fluorescence in-situ hybridiztion (FISH) to detect the integration of HBV on PBMC chromosome of pregnant women. Methods 136 HBV carrier mothers and 28 healthy women in our department were selected to set up the PBMC chromosome specimens. Chromosome in-situ hybridization was done with Dig-labeled HBV probe. Results Green fluorescent signals were seen in 4 patient specimens ( 2.94%). All four patients possessed all positive HBsAg and HBeAg mothers. The integration sites were present in low frequency and randomly distributied. There were no positive singals in HBsAg(+)/HBeAg(-) group and control group. Conclusion Using fluorescence in-situ hybridization technique could detect HBV integration on the PBMC chromosome. HBV integrating into PBMC chromosome of pregnant women not only can lead to vertical transmission, but also may result in genetic heredity, which is a high risk factor for hereditary diseases and liver cancer.