氧化苦参碱对脂多糖诱导的RAW264.7细胞一氧化氮及其诱导型合酶表达的影响

目的：观察氧化苦参碱（OMT））对脂多糖（LPS）诱导的RAW 264.7细胞一氧化氮（NO）释放量及诱导型一氧化氮合酶（iNOS）表达的影响。方法：采用LPS诱导RAW 264.7细胞建立细胞炎症反应模型。实验分组：空白对照组、LPS组（1μg/mL）、OMT组（100μmol/L）、LPS＋OMT小剂量组（20μmol/L）、LPS＋OMT中剂量组（50μmol/L）、LPS＋OMT大剂量组（100μmol/L）。采用Griess试剂法测定细胞培养液中NO释放量。采用逆转录聚合酶链式反应（RT-PCR）方法测定RAW 264.7细胞iNOS mRNA的表达。结果：OMT显著减少LPS诱导的RAW 264.7细胞NO的释放（P〈0.05,P〈0.01）;同时减少LPS诱导的RAW 264.7细胞iNOS mRNA的表达（P〈0.05,P〈0.01）。结论：OMT可能通过抑制LPS诱导的巨噬细胞iNOS mRNA表达,减少NO的释放量而发挥抗炎作用。

Effect of oxymatrine on lipopolysaccharide-induced nitric oxide releasing and inducible nitric oxide synthase expression in RAW 264.7 cells

Objective： To investigate the effect of Oxymatrine on nitric oxide（NO） releasing and inducible nitric oxide synthase（iNOS） expression in lipopolysaccharide（LPS）-induced macrophage cells（RAW 264.7）.Methods： LPS-activated RAW 264.7 cells was employed as an inflammation model.RAW 264.7 cells were divided into 6 groups（n=6）： control group,LPS group（1 μg/mL）,OMT group（100 μmol/L）,LPS＋OMT small dosage group（20 μmol/L）,LPS＋OMT middle dosage group（50 μmol/L）,LPS＋OMT high dosage group（100 μmol/L）.The production of NO was examined by Griess reaction,and the expression of iNOS mRNA was detected by RT-PCR.Results： OMT decreased the production of NO induced by LPS（P〈0.05,P〈0.01） and also suppressed LPS-induced iNOS mRNA expression in RAW 264.7 cells（P〈0.05,P〈0.01）.Conclusion： OMT presents an anti-inflammatory effect by inhibiting iNOS level and reducing NO production.