兔骨髓间充质干细胞移植治疗心肌梗死的实验研究

目的:探讨骨髓间充质干细胞(mesenehymal stem cells,MSCs)移植对兔心肌梗死(myoeardial infarction,MI)的治疗作用。方法:大耳白兔38只,2只用于提供MSCs,余36只随机分为对照组、MI组和MSCs移植组。采用结扎左冠状动脉前降支方法制作MI模型,在MI后1小时将DAPI标记的MSCs移植至梗死区,对照组注射等量PBS。术前、术后2周、4周分别做超声心动图检查其心功能变化。术后4周处死兔.取心梗区组织进行组织学观察。结果:心梗4周后,MSCs组心肌组织冷冻切片中可以观察到DAPI标记细胞存在。MSCs组心功能和毛细血管密度与MI组比较差异均有统计学意义(P<0.05)。结论:经5-aza诱导的MSCs同种异体移植入兔MI模型的受损心肌后能存活并改善宿主的心功能。

An experimental study of bone marrow mesenchymal stem cells transplantation to treat myocardial infarction in rabbits

Objective:To investigate the effects of mesenchymal stem cells (MSCs) transplantation on the therapy of myocar- dial infarction (MI) in rabbits.Methods:36 rabbits were randomly divided into three groups:the control group,the MI group,the MSCs group.MI was induced by ligation of left anterior descending branch of the coronary artery.The MSCs labeled by DAPI were transplanted after MI 1 h.Echocardiography was performed to evaluate the cardiac function before operation and 2 weeks and 4 weeks after MI.After four weeks,specimens were harvested from the transplanted areas and observed histologically.Re- sults:Four weeks after AMI,the labeled cells were viable in the recipient heart.In contrast with the MI group,the heart function and vascular density of the MSCs group had significant difference (P<0.05).Conclusion:The rabbit MSCs induced by 5-aza and later implanted into the rabbit injured cardiac muscle can survive and improve the host's cardiac function.