“正交法”与药典法姜半夏中成分含量及对动物作用的比较

目的：比较“正交法”和药典法两种炮制工艺炮制的姜半夏中成分的含量及两种炮制品对动物的作用。方法：总生物碱含量测定采用重量法、酸性染料比色法；浸出物、灰分测定采用药典附录法；毒性采用LD₅₀测试；胃液分泌采用大鼠幽门结扎，以紫外分光光度法测定PGE₂含量，酸度计测试胃酸，改良Ｍｅｔ法测定胃蛋白酶活性。结果：总生物碱含量以“正交法”较高；灰分、浸出物含量以药典法较高；两种姜半夏混悬液、水煎液灌胃均未见明显毒性，但水煎液腹腔注射以药典法毒性较大，其LD₅₀为（１３．３４６±０．０１９）ｇ／ｋｇ；两种姜半夏对大鼠胃液中PGE₂含量、胃蛋白酶活性均呈促进作用，但对胃液量无明显影响。结论：两种工艺炮制的姜半夏无明显差异，以“正交法”炮制品较好。

The Comparison of Component Contents and Pharmacological Actions Between Two Kinds of Processed Products of Pinellia Rhizoma Prepared by Ginger Juice and Alum

Objective:To compare the component contents in Pinellia Rhizoma processed with ginger separately on orthogonal technology and pharmaceutical technology stipulated in pharmacopoeia,and study the action of the two differently processed products on animals.Mehtod:Using gravimetric method and acid dye colorimetry to determine the total alkaloids,et al.Result and Conclusion:The experiment has shown that the contents of total alkaloids of product of Pinellia Rhizoma processed by orthogonal technology are higher than those processed by pharmaceutical technology,but the contents of digged substances of the latter are higher than those of the former, and the toxicity of decoction of the latter is also higher than that of the former.Compared with raw Pinellia Rhizoma,the two products of Pinellia Rhizoma prepared by different technologies all have shown promoting action on PGE 2 contents and activity of gastric proteinase in rat gastric juice.The experimental results also have shown that on the above mentioned items.There are no obvious differences between the product of Pinellia Rhizoma processed by orthogonal technology and the product processed by pharmaceutical technology.However,the component contents of raw Pinellia Rhizoma are lower than those of the above mentioned two processed products and help inhibit PGE 2 contents and activity of gastric proteinase in rat gastric juice.