| 乙型肝炎病毒1896位点变异荧光PCR法检测及意义 |
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| 引用本文： | 李秀梅,汤华.乙型肝炎病毒1896位点变异荧光PCR法检测及意义[J].天津医科大学学报,2010,16(2):337-338,344. |
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| 作者姓名： | 李秀梅 汤华 |
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| 作者单位： | 1. 天津医科大学基础医学院,天津市生命科学中心实验室,天津,300070天津市传染病医院
2. 天津医科大学基础医学院,天津市生命科学中心实验室,天津,300070 |
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| 摘 要： | 目的：探讨乙型肝炎病毒（HBV）前C区1896位点突变与临床关系。方法：采用荧光聚合酶链反应（PCR）对74例各型乙肝患者HBV前C区基因进行扩增，鉴定前C区1896位点突变。结果：有41例（55．41％）发生了1896位点突变。其中慢性乙型肝炎（CHB）和肝硬化（LC）组均高于无症状HBV携带者组（ASC）（P〈0．05）。抗-HBe（＋）组的变异检出率为71．11％，高于HBeAg（＋）组的31．03％（P〈0．01）。并且随着HBVDNA含量的增高，其发生变异的可能性越大（P〈0．01）。结论：前C区1896位点突变的发生可能与机体长期的炎症活动、免疫压力的选择有关。
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| 关 键 词： | 乙型肝炎病毒 脱氧核糖核酸 基因变异 聚合酶链反应，荧光 |
Detection of serum hepatitis B virus 1896 point mutation in hepatitis B patients by fluorescent PCR |
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| LI Xiu-mei,TANG Hua.Detection of serum hepatitis B virus 1896 point mutation in hepatitis B patients by fluorescent PCR[J].Journal of Tianjin Medical University,2010,16(2):337-338,344. |
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| Authors: | LI Xiu-mei TANG Hua |
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| Institution: | 1.Basic Medical College ,Tianjin Medical University,Tianjin Life Science Research Centre Laboratory, Tianjin 300070, China; 2.Tianjin Infectious Disease Hospital) |
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| Abstract: | Objective： To discuss the relationship between the HBV pre-C 1896 point mutation and clinic. Methods： HBV pre-C region was detected by fluorescent PCR in serum of 74 patients with HBV to identify 1896 point mutation. Results： Forty-one of them （55.41%） had the pre-C 1896 point mutation. The pre-C 1896 point mutation rates in chronic hepatitis B （CHB） and liver cirrhosis （LC） were higher than that in asymptomatie HBV carries （ASC） （P〈0.05）.The rate was higher in anti-HBe positive group（71.11%） than that in HBeAg positive group （31.03%） （P〈0.01）. The possibility of mutations increased along with the rise of HBV DNA quantities （P〈0.01）. Conclusion： The pre-C 1896 point mutation may be related to the long term inflammation of liver and the immune pressure. |
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| Keywords: | Hepatitis B virus DNA Mutation Polymerase chain reaction fluorescence |
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