银染端粒重复序列扩增法定量检测端粒酶活性的研究

目的：探讨运用简便、快速及无放射性污染的银染粒重复序列扩增法（silver staining telomeric repeat amplification protocol,SS-TRAP）定量检测端粒酶活性。方法：运用SS－TRAP法检测了293细胞、经加热处理的293细胞、空白对照及典型乳腺癌组织、良性乳腺病变标本中端粒酶活性，其结果予以定量。结果：该法有检测出10个以上293细胞中端粒酶活性，热处理及空白对照均为阴性；乳癌组织标本中银染条带清晰可见，而良性病变中未见端粒酶阳性条带。结论：非核素银染TRAP法可用于端粒酶活性定量检测，与TRAP法相比，同样具有较高敏感性和特异性，但更快速、简便。

STUDY ON QUANTITATIVE DETECTION OF TELOMERASE ACTIVITY BY SILVER STAINING TELOMERASE REPEAT AMPLIFICATION PROTOCOL

Objective: To investigate the quick and easy method of quantitative detection of telomerase activity by non-radioisotopic contaminative telomerase repeat amplification protocol (TRAP). Methods: Telomerase activity in telomerase positive 293 cell?heat-treated 293 cell?blank control?typical breast carcinoma tissues and galactophore benign lesions was detected by silver staining TRAP, and the results were quntitated. Results: At least ten of 293 cells were telomerase positive, telomerase activity was not detected in heated-treated and blank control, silver staining bands of breast carccinoma specimen were clear, but telomerase possitive band was not observed in benign lesions. Conclusion: Compared with TRAP assay, non-radioisotopic silver staining TRAP has the same sensitivity and specificity for quantitative detection of telomerase actiivity, but it can be performed more eassily and much faster. It deserves to be popularized in further clinical application.