Survivin与Bax蛋白在宫颈上皮内瘤变和宫颈癌中的表达及临床意义

目的研究Survivin、Bax在宫颈上皮内瘤变(CIN)及宫颈癌中的表达,探讨二者在宫颈癌发生、发展机制中的作用及临床意义。方法采用免疫组织化学法检测Survivin和Bax蛋白在83例宫颈上皮内瘤变及71例宫颈癌中的表达,另取12例正常宫颈组织作为对照。结果 Survivin蛋白的阳性率在宫颈癌、宫颈上皮内瘤变和正常宫颈组织中分别为76.1%、45.8%和0%,Bax蛋白的阳性表达率分别为7%、39.8%和75.0%(P<0.05)。Survivin蛋白表达在CINⅢ高于CINⅡ,CINⅡ高于CINⅠ,在宫颈癌Ⅲ期高于Ⅱb期,Ⅱb期高于Ⅰ~Ⅱa期,在淋巴结转移组高于无淋巴结转移组(P<0.05)。在宫颈癌与CIN中Survivin与Bax的表达呈负相关。结论 Survivin和Bax的异常表达可能参与宫颈癌的发生、发展过程,在抗凋亡作用增强、促凋亡作用下降两方面协同发挥作用,二者的表达程度与不良预后有关。

Expression and Clinical Significance of Surviving and Bax in Cervical Intraepithelial Neoplasia and Cervical Carcinoma

Objective To investigate the expression of surviving and Bax in cervical intraepithelial neoplasia and cervical carcinoma,and to explore the role of them in cervical carcinoma pathogenesis.Methods The exprssion of Survivin and Bax was detected with immunohistochemical method in situ in CIN and cervical carcinoma.Normal cervical tissues were acted as the control.Results The positive rates of Survivin protein in cervical carcinoma,CIN and the normal cervical tissues were 76.1%,45.8% and 0%,respectively.While the expression of Bax were 7%,39.8% and 75.0% in the above three groups（P0.05）.The expression of Survivin in CIN Ⅲ was obviously more significant than those in CIN Ⅰ/Ⅱ.And the differences were obviously more significant in cervical carcinoma at stage Ⅱb-Ⅲ than those at stage Ⅰ~Ⅱa.The expression of Survivin in cervical carcinoma with lymphatic metastasisi was more significant difference than those with lymphatic metastasis.Negative correlation between Survivin and Bax protein was observed in cervical carcinoma.Conclusion The abnormal expression of Survivin and bax may participate in the occurrence and development of cervica carcinoma,and suggest malignant degree of tumor and patients＇ poor prognosis.