HPLC法同时测定繁枝补血草中槲皮苷和槲皮素的含量

目的建立同时测定繁枝补血草中槲皮苷和槲皮素含量的HPLC法。方法采用Agilent C18色谱柱(250mm×4.6mm,5.0μm);流动相为甲醇-4mL·L-1磷酸水溶液(50∶50);流速为1.0mL·min-1;检测波长为360nm;柱温为25℃;进样量为10μL。用外标法计算其含量。结果槲皮苷质量浓度在4.912~98.24μg·mL-1范围内与峰面积有良好的线性关系,回归方程y=30.434x-11.864(r=0.999 6);槲皮素质量浓度在12.833~102.667μg·mL-1范围内与峰面积有良好的线性关系,回归方程y=43.358x-45.586(r=0.999 7)。槲皮苷的平均回收率为100.73%,RSD值为1.82%(n=6);槲皮素的平均回收率为100.47%,RSD值为1.35%(n=6)。结论该方法操作简便、快速、准确、误差小、重复性好,可作为繁枝补血草中槲皮苷和槲皮素的定量分析方法。

Simultaneous determination of the content of quercitrin and quercetin in Limonium myrianthum (Schrenk) O.Kuntze by HPLC

Objective To establish an HPLC method for the determination of quercitrin and quercetin in Limonium myrianthum (Schrenk) O.Kuntze.Methods Quercitrin and quercetin in Limonium myrianthum (Schrenk) O.Kuntze were determined by HPLC using Agilent C18 column (250 mm×4.6 mm,5.0 μm) at 360 nm of detection wavelength.The mobile phase was methanol-4 mL·L-1phosphoric acid solution (50∶50), the flow rate was 1.0 mL·min-1 , the column temperature was 25 ℃,and the injection volume was 10 μL.Results The linear ranges of quercitrin and quercetin were 4.912-98.24 and 12.833-102.667 μg·mL-1, respectively.The regression equations were y=30.434x-11.864,r=0.999 6 and y=43.358x-45.586,r=0.999 7, respectively.The average recoveries were 100.73% and 100.47% respectively,and RSD was 1.82%(n=6),1.35%(n=6),respectively.Conclusion The developed method is simple,rapid,and accurate with good reproducibility,and therefore suitable for the quantitative analysis of quercitrin and quercetin in Limonium myrianthum (Schrenk) O.Kuntze.