| 聚肌胞对RSV感染哮喘加重小鼠气道分泌TSLP和炎症的影响 |
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| 引用本文： | 夏虎,于化鹏,骆利敏,蔡绍曦.聚肌胞对RSV感染哮喘加重小鼠气道分泌TSLP和炎症的影响[J].南方医科大学学报,2011,31(3):434-437. |
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| 作者姓名： | 夏虎 于化鹏 骆利敏 蔡绍曦 |
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| 作者单位： | 夏虎,于化鹏,XIA Hu,YU Hua-peng(南方医科大学珠江医院呼吸内科,广东,广州,510282);骆利敏,LUO Li-min(解放军第458医院传染科,广东,广州,510602);蔡绍曦,CAI Shao-xi(南方医科大学南方医院呼吸内科,广东,广州,510515) |
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| 基金项目： | 国家自然科学基金，广东省医学科研基金 |
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| 摘 要： | 目的探讨聚肌胞(poly I:C)对呼吸道合胞病毒(RSV)感染哮喘加重小鼠气道分泌胸腺基质淋巴细胞生成素(TSLP)和气道炎症的影响。方法雌性BALB/c小鼠32只,随机分成4组:分别为磷酸盐缓冲液(PBS)对照组、鸡卵白蛋白(OVA)组、OVA/RSV组、OVA/RSV/polyI:C组;应用OVA腹腔注射致敏、OVA气道雾化结合RSV滴鼻激发哮喘,聚肌胞1mg/kg肌肉注射;无创肺功能检测各组小鼠气道反应性;ELISA法检测小鼠血清IL-4、IL-5、IL-13、IFN-γ水平;ELISA法检测气管灌洗液(BALF)中TSLP含量;BALF行细胞分类计数;病理观察小鼠肺组织炎症反应,免疫组化观察小鼠气道上皮细胞TSLP表达水平。结果无创肺功能检测显示聚肌胞抑制RSV感染哮喘加重小鼠气道反应性的增高,OVA/RSV/polyI:C组小鼠Penh值明显低于OVA/RSV组(P<0.01);OVA/RSV/polyI:C组小鼠血清IL-4、IL-5、IL-13和BALF中TSLP浓度均明显低于OVA/RSV组(P<0.05);OVA/RSV/poly I:C组小鼠BALF细胞总数、嗜酸性粒细胞数及淋巴细胞数明显少于OVA/RSV组(P<0.05);病理观察显示聚肌胞减轻RSV感染哮喘小鼠气道炎症细胞浸润;免疫组化染色证实聚肌胞抑制RSV感染哮喘小鼠气道上皮细胞TSLP表达。结论聚肌胞前期注射减少RSV感染哮喘加重小鼠气道上皮细胞表达TSLP,抑制哮喘小鼠气道炎症反应。
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| 关 键 词： | 聚肌胞 胸腺基质淋巴细胞生成素 呼吸道合胞病毒 哮喘 |
Effect of polyI:C on secretion of thymic stromal lymphopoietin and airway inflammation in mice with respiratory syncytial virus-induced asthma exacerbation |
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| XIA Hu,YU Hua-peng,LUO Li-min,CAI Shao-xi.Effect of polyI:C on secretion of thymic stromal lymphopoietin and airway inflammation in mice with respiratory syncytial virus-induced asthma exacerbation[J].Journal of Southern Medical University,2011,31(3):434-437. |
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| Authors: | XIA Hu YU Hua-peng LUO Li-min CAI Shao-xi |
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| Institution: | XIA Hu1,YU Hua-peng1,LUO Li-min2,CAI Shao-xi3 1Department of Respiratory Disease,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China,2Department of Infectious Disease,458 Hospital of PLA,Guangzhou 510602,3Department of Respiratory Disease,Nanfang Hospital,Guangzhou 510515 |
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| Abstract: | Objective To investigate the effects of polyinosinic-polycytidylic acid(polyI:C) on the production of thymic stromal lymphopoietin(TSLP) and airway inflammation in mice with exacerbated asthma induced by respiratory syncytial virus(RSV).Methods Thirty-two female BALB/c mice were randomly divided into 4 groups,namely the PBS control group,OVA group,OVA/RSV group,and OVA/RSV/polyI:C group.In the latter 3 groups,the mice were sensitized by OVA and stimulated with nebulized OVA.RSV was inoculated into the nasal... |
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| Keywords: | polyI:C thymic stromal lymphopoietin respiratory syncytial virus asthma |
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