BDNF、TrkB在岛叶慢性电点燃大鼠海马中的表达及与学习记忆的关系

目的研究岛叶慢性电点燃癫痫模型大鼠的学习记忆功能,检测脑源性神经营养因子（brain-derivedneurotrophic factor,BDNF）、酪氨酸蛋白激酶受体B（Tyrosine receptor kinase B,TrkB）在海马组织中表达,探讨BDNF、TRKB与岛叶点燃大鼠学习记忆的关系。方法成年SD大鼠随机分为模型组、假手术组、空白对照组,建立大鼠岛叶慢性电点燃模型。用避暗试验方法检测岛叶慢性电点燃模型大鼠学习记忆功能,采用免疫组化和western blot方法分别检测BDNF、TrkB蛋白在海马组织中表达水平,用RT-PCR检测两因子基因表达水平。结果岛叶慢性电刺激点燃成功后第1天（即0周）,学习记忆能力点燃组与空白对照组、假手术组差异无统计学意义（P〉0.05）。点燃2周点燃组避暗试验结果的LT为（48.87±3.42 s）、EN为（2.75±0.99次）;点燃3周点燃组避暗试验结果的LT为（48.71±2.77 s）、EN为（2.75±1.15次）,与点燃当天（即点燃0周）相比学习记忆功能增强（P〈0.05）,点燃4周后与对照组比学习记忆功能明显下降（P〈0.05）。BDNF、TrkB表达均在24h达到高峰（P〈0.05）,空白对照组、假手术组无明显变化。组间比较、峰值及增幅均出现在2～3周内（P〈0.01）,4周后较前几周组明显下降（P〈0.05）。结论岛叶电点燃大鼠学习记忆功能与BD-NF、TrkB在海马中表达总变化趋势一致。BDNF、TrkB与岛叶点燃大鼠学习记忆功能密切相关。

The Expression of BDNF and TrkB in Rat Hippocampus and Its Relationship with Learning-remembering Ability in Chronic Electronic Ignition of Insular Lobe

Objective To investigate the expression of active Skeleton Adjustment Protein in rat's Hippocampus in the model of insular chronic electricity-igniting epilepsy and to explore the relation between BDNF,TrkB and learning-remembering ability in the epileptic rat.Methods Adult SD rats were randomly divided into model,sham and control group.The Passive avoidance test was employed to test learning-remembering ability of rats.Immunohistochemistry and western blot was used to determine the expression of BDNF,TrkB ...