3′-甲氧基葛根素对大鼠脑缺血保护作用研究

目的:探讨中药葛根提取物3′-甲氧基葛根素(3′-methoxy-puerarin,3′-mo-pue)的脑保护作用,为扩大葛根的应用范围提供实验依据。方法:将动物随机分为假手术组、模型组、葛根素(puerarin,Pue)组和3′-mo-pue组,采用三氯化铁覆着大脑中动脉造成大鼠局灶性脑缺血模型(MCAO);采用大鼠双侧颈总动脉结扎造成脑缺血再灌模型;观察不同剂量(0.1,0.2,0.4g.kg^-1)的3′-mo-pue对脑缺血的影响。结果:3′-mo-pue可不同程度的降低MCAO模型大鼠神经行为学评分、脑梗死范围和脑含水量;显著提高脑缺血再灌模型大鼠缺血区海马过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活性和皮层CAT活性;降低脑缺血再灌模型大鼠缺血区海马过氧化脂质(LPO)和乳酸(LD)的含量。结论:3′-mo-pue具有脑保护作用,其机制可能与其抗氧化作用有关。

Protective effects of 3'-methoxy-puerarin on rat brain suffering from ischemia

Objective:To research on the protective effect of 3'-methoxy-puerarin(3'-mo-pue)on rat brain suffering from ischemia and to offer the experimental basis for widening the applied areas of Radix Puerariae.Method:All rats were randomly divided into sham operation group,control group,Pue group and 3'-mo-pue group.The influence of different dosage on relevant indexes were measured through cerebral artery occlusion(MCAO)model rat covered by Fecl₃,and cerebral ischemia-reperfusion model rat after 2VO.Result:The result showed that 3'-mo-Pue could reduce the scores of neurological deficit,cerebral infarcted zone to varying degrees and the water content of brain tissue in MCAO model.It could obviously increase the activity of CAT and GPx in the hippocampus and also increase the activity of CAT and GPx in the cortex in the ischemia field.Moreover,3'-mo-Pue could decrease the content of LPO,LD in the brain tissue of cerebral ischemia-reperfusion model rat.Conclusion:3'-mo-Pue has favorable protective effect on brain suffering from ischemia.The mechanism is possibly related to its effect of anti-oxidation.