| pH值对IFN-α2b、rIL-2、ADM合用维拉帕米对人肝癌细胞杀伤作用的影响 |
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| 引用本文： | 范平生,李继德,王晓琰,胡冰,林新民,庄建生.pH值对IFN-α2b、rIL-2、ADM合用维拉帕米对人肝癌细胞杀伤作用的影响[J].实用全科医学,2003,1(1):16-18. |
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| 作者姓名： | 范平生 李继德 王晓琰 胡冰 林新民 庄建生 |
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| 作者单位： | 安徽省立医院分院，安徽省立医院分院，安徽省立医院分院，安徽省立医院肿瘤科，安徽省立医院肿瘤科，安徽省立医院肿瘤科 230031合肥市，230031合肥市，230031合肥市 |
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| 基金项目： | 安徽省重点科研项目资助项目(0 10 2 30 2 7) |
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| 摘 要： | 目的 观察培养液在 pH值为 6 .8、7.3、7.6条件下rIL - 2、IFN -α2b、ADM及合用维拉帕米对人肝癌细胞 74 0 4杀伤作用的影响。方法 MTT法于 96孔培养板上进行杀伤实验 ,测定培养液pH值分别为 6 .8、7.3、7.6状态下 ,rIL - 2、IFN -α2b、ADM、及与维拉帕米联合杀伤人肝癌细胞 70 4 0的差异。结果 pH值为 7.6状态下rIL - 2、IFN -α2b、ADM及合用维拉帕米杀伤效果最佳。pH值为 6 .8、7.3、7.6时 ,IFN -α2b都能增加ADM抗肿瘤作用 ,但pH值为 7.6时 ,IFN -α2b +ADM杀伤效果最佳。结论 在偏硷性环境下 ,rIL - 2、IFN -α2b、ADM及合用维拉帕米对肿瘤细胞杀伤效果最佳
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| 关 键 词： | 干扰素 阿霉素 维拉帕米 肿瘤细胞 弱硷性 |
| 修稿时间： | 2003年1月13日 |
Effect of pH on the Lethality of rIL - 2, IFN - α2b, ADM and Combination with Verapamil Against Human Hepatic Carcinoma Cells |
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| Fan Pingsheng,Li Jide,Wang Xiaoyan,Hu Bing,Lin Xinmin,Zhuang Jiansheng.Branch of Anhui Provincial Hospital Hefei,Anhui.Effect of pH on the Lethality of rIL - 2, IFN - α2b, ADM and Combination with Verapamil Against Human Hepatic Carcinoma Cells[J].Applied Journal Of General Practice,2003,1(1):16-18. |
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| Authors: | Fan Pingsheng Li Jide Wang Xiaoyan Hu Bing Lin Xinmin Zhuang JianshengBranch of Anhui Provincial Hospital Hefei Anhui |
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| Institution: | Fan Pingsheng,Li Jide,Wang Xiaoyan,Hu Bing,Lin Xinmin,Zhuang Jiansheng.Branch of Anhui Provincial Hospital Hefei,Anhui 230031 |
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| Abstract: | Objective To observe and determine the alteration of the lethality of rlL - 2 ,IFN - a2b,ADM and combination with verapamil against human hepatic carcinoma cells when pH is 6.8, 7.3, and 7.6. Method By means of MTT technique, perform killing experiments on 96 - hole culture plates , determine the difference of lethality of rlL - 2, IFN - a2b, ADM, combination with verapamil against human hepatic carcinoma cells when pH of the culture is respectively 6.8,7.3 and 7.6. Result rlL- 2,IFN- o.2b, ADM and combination with verapamil have maximum lethality when pH is 7.6. Under all of the three pH values, but IFN - a2b can strengthen the anti-tumor role of ADM, IFN - a2b + ADM yield optimal lethality when pH is 7.6. Conclusion rIL - 2,IFN - a2b,ADM and combination with verapamil are most effective in killing tumor cells under weakly alkaline condition. |
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| Keywords: | Interferon Adriamycin Verapamil Tumor cellsm Weak alkalinity |
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