| 大鼠局灶性脑缺血模型评价方法间关联性分析 |
| |
| 引用本文： | 崔煦然,刘钊,张志斌,钟菊迎.大鼠局灶性脑缺血模型评价方法间关联性分析[J].实验动物与比较医学,2015,23(5):506-508,512. |
| |
| 作者姓名： | 崔煦然 刘钊 张志斌 钟菊迎 |
| |
| 作者单位： | 中国中医科学院医学实验中心,中国中医科学院医学实验中心,帕瑞医学科技（北京）有限公司,中国中医科学院医学实验中心 |
| |
| 基金项目： | 中国中医科学院院自主课题(ZZ2012012)。 |
| |
| 摘 要： | 目的 分析评价线栓法制备大鼠大脑中动脉阻塞模型成功的三种方法之间的相关性，为评价该模型提供一种新的方法和标准。 方法 雄性SD大鼠共（30）只，随机分成假手术组(n=15)、模型组（n=15），参照Zea-longa线栓法稍加改进制备局灶性脑缺血模型，于术前术后检测各组大鼠脑血流变化。以大鼠脑梗塞面积率为金标准，分析该标准与脑血流变化之间的相关性。 结果 模型大鼠脑梗塞面积率与脑血流变化量呈正相关，与行为学评分高低无关。结论 大鼠脑血流量变化可作为评价局灶性脑缺血模型成功的标准。
|
| 关 键 词： | 脑梗死面积 行为学评分 脑血流量 大鼠 |
| 收稿时间： | 2015/5/12 0:00:00 |
| 修稿时间： | 2015/6/24 0:00:00 |
Analysis of the correlation between three evaluation methods of rat models of focal cerebral ischemia/reperfusion |
| |
| CUI Xu-ran,LIU Zhao,ZHANG Zhi-bin and ZHONG Ju-ying.Analysis of the correlation between three evaluation methods of rat models of focal cerebral ischemia/reperfusion[J].Laboratory Animal and Comparative Medicine,2015,23(5):506-508,512. |
| |
| Authors: | CUI Xu-ran LIU Zhao ZHANG Zhi-bin and ZHONG Ju-ying |
| |
| Institution: | China Academy of Traditional Chinese Medicine Experimental Center for Molecular Biology Laboratory, State Key Laboratory of Medical Research Center of Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China;China Academy of Traditional Chinese Medicine Experimental Center for Molecular Biology Laboratory, State Key Laboratory of Medical Research Center of Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China;China Academy of Traditional Chinese Medicine Experimental Center for Molecular Biology Laboratory, State Key Laboratory of Medical Research Center of Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China;China Academy of Traditional Chinese Medicine Experimental Center for Molecular Biology Laboratory, State Key Laboratory of Medical Research Center of Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China |
| |
| Abstract: | Objective To analyze the correlation between three evaluation methods of middle cerebral artery occlusion and focal cerebral ischemia, and to explore a new method and standard for the evaluation of the model. Methods Thirty healthy adult male SD rats were randomly divided into a sham-operated group (n=20) and model group. According to Zea-Longa procedure,we established the disease model to detect the changes in cerebral blood flow before and after surgery. The resulted cerebral infarction area was taken as gold standards. Then we analyzed the correlation between the standards and the changes in cerebral blood flow. Results The changes of infarction area ratio were positively correlated with the changes of cerebral blood flow in the model rats. Conclusions The changes of rat cerebral blood flow can be used to evaluate whether the cerebral ischemia model is successful or not. |
| |
| Keywords: | Infarct area Behavioral score Cerebral blood flow Rats |
|
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
| 点击此处可从《实验动物与比较医学》下载免费的PDF全文 |
|
