| 葛根素对缺氧缺血新生大鼠神经保护作用及其机制 |
| |
| 引用本文： | 许蓓,肖农,张晓萍.葛根素对缺氧缺血新生大鼠神经保护作用及其机制[J].第四军医大学学报,2009(23):2757-2760. |
| |
| 作者姓名： | 许蓓 肖农 张晓萍 |
| |
| 作者单位： | 重庆医科大学附属儿童医院康复中心;重庆医科大学附属儿童医院儿科研究所; |
| |
| 基金项目： | 重庆市卫生局科研项目(渝卫科教[2007]1号-07-2-153) |
| |
| 摘 要： | 目的：观察葛根素（Pue）对缺氧缺血性脑损伤（HIBD）新生大鼠脑源性神经生长因子（BDNF）,Bax,及半胱天冬酶-3表达及远期学习记忆能力的影响.方法：7d龄S D大鼠随机分为3组：葛根素组（Pue组）在缺氧缺血后注射葛根素50 mg/kg,1次/d,共7 d;对照组（Con组）给予同体积的的生理盐水;假手术组（Sham组）仅分离经总动脉,不给药.各组分别于1 wk后采用免疫组化方法检测各组BDNF,Bax,Caspase-3在海马区的表达,4 wk后水迷宫检测其远期学习记忆能力.结果：①术后1 wk海马区Pue组BDNF表达水平高于Con组（P〈0.05）,海马区Bax,Caspase-3表达水平低于Con组（P〈0.05,P〈0.01）.②术后4 wk Morris水迷宫Pue组逃避潜伏期缩短明显快于Con组（P〈0.05,P〈0.01）,穿过原平台次数及在目标象限游泳时间与总时间百分比Pue组明显多于Con组（P〈0.05,P〈0.01）.结论：葛根素能减轻新生大鼠缺氧缺血引起的脑组织损伤,提高新生大鼠年长后的学习记忆能力.这种保护作用可能和葛根素增加海马区BD-NF的表达、降低Bax,Caspase-3在海马区的表达抑制海马区神经细胞的凋亡有关.
|
| 关 键 词： | 葛根素 缺氧缺血 脑源性神经生长因子 半胱天冬酶-3 Bax 水迷宫 |
Neuroprotective effect of puerarin after hypoxic-ischemic in neonatal and its mechanism |
| |
| XU Bei,XIAO Nong,ZHANG Xiao-Ping.Neuroprotective effect of puerarin after hypoxic-ischemic in neonatal and its mechanism[J].Journal of the Fourth Military Medical University,2009(23):2757-2760. |
| |
| Authors: | XU Bei XIAO Nong ZHANG Xiao-Ping |
| |
| Institution: | XU Bei1,XIAO Nong1,ZHANG Xiao-Ping2 1Department of Rehabilitation Center,2Pediatric Research Institute,Children's Hospital of Chongqing Medical University,Chongqing 400014,China |
| |
| Abstract: | AIM:To investigate the effects of puerarin on the expression of BDNF,Bax,Caspase-3 in hippocampal cell and impact on learning and memory ability after hypoxia-ischemia brain injury in neonatal rats.METHODS:All SD rats(P7) were randomly divided into 3 groups:treatment with Puerarin group,control group,and sham group.Rats in Puerarin group and control received hypoxia-ischemia brain injury.Rats in Puerarin group were treated with Puerarin in doses of 50 mg/kg ip at 5 min after reoxygenation,once a day for 7 d... |
| |
| Keywords: | puerarin hyposic-ischemic BDNF Caspase-3 Bax morris water maze |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
