| RP-HPLC测定舒他兰锌及其有关物质 |
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| 引用本文： | 刘宏,谭生建,王建,黄君曦,薛金萍,黄淑霞,陈耐生,黄金陵.RP-HPLC测定舒他兰锌及其有关物质[J].中国药学杂志,2009,44(1):59-62. |
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| 作者姓名： | 刘宏 谭生建 王建 黄君曦 薛金萍 黄淑霞 陈耐生 黄金陵 |
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| 作者单位： | 1.福州大学功能材料研究所 福州 350002;2.中国人民解放军306医院 北京 100101;3.福建省龙华药业有限责任公司 福州 350008 |
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| 基金项目： | 国家重点科技项目(攻关)计划专题(96-901-01-67);福建省科技厅重大项目(2001H023);福建省科技厅重点项目(2006Y0020)资助 |
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| 摘 要： | 目的建立反相高效液相色谱法测定抗癌光敏剂舒他兰锌(Suftalan zinc)含量及有关物质。方法采用SHIMADZU SHIM-PACK VP-ODS色谱柱(4.6 mm×150 mm,5μm),以四氢呋喃-甲醇-N,N二甲基甲酰胺-水-三乙胺(150∶105∶755∶1 000∶1.4,磷酸调pH值6.5～7.0)为流动相,流速1.0 mL·min-1,紫外可见检测器于677 nm测定。结果RP-HPLC测定的线性范围为20.0～120.1 mg·L-1,相关系数r=0.999 9;最低检测限为0.048μg;样品溶液至少在3 d内稳定;日内精密度(RSD=0.70%)良好。结论采用反相高效液相色谱法测定抗癌光敏剂(舒他兰锌)含量及有关物质方法简便,结果准确。
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| 关 键 词： | 舒他兰锌 含量测定 反相高效液相色谱法 |
| 收稿时间： | 2008-01-11; |
Determination of Suftalan Zinc and Its Related Substances by RP-HPLC Assays |
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| LIU Hong,TAN Sheng-jian,WANG Jian,HUANG Jun-xi,XUE Jin-ping,HUANG Shu-xia,CHEN Nai-sheng,HUANG Jin-ling.Determination of Suftalan Zinc and Its Related Substances by RP-HPLC Assays[J].Chinese Pharmaceutical Journal,2009,44(1):59-62. |
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| Authors: | LIU Hong TAN Sheng-jian WANG Jian HUANG Jun-xi XUE Jin-ping HUANG Shu-xia CHEN Nai-sheng HUANG Jin-ling |
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| Institution: | 1.Institute of Functional Material，Fuzhou University，Fuzhou 350002，China；2. 306th Hospital of PLA，Beijing 100101，China；3. Fujian Longhua Pharmaceutical Co.，Ltd. Fuzhou 350008，China |
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| Abstract: | OBJECTIVE To establish a RP-HPLC method for the determination Suftalan zinc and its related substances.METHODS A separation was performed on a SHIM-PACK VP-ODS column(4.6 mm×150 mm,5 μm) and mobile phase was THF-CH3OH-DMF-H2O-TEA(150∶105∶755∶1 000:1.4,adjust pH to 6.5-7.0 using H3PO4) with a flow rate of 1 mL·min-1.The detective wavelength was 677 nm.RESULTS The calibration curve was linear in the concentration range of 20.0～120.1 mg·L-1(r=0.999 9).The LOD was 0.048 μg.The sample solution was stable within 3 d,intra-day validation(RSD=0.70%) was good.CONCLUSION The method to determine the content and its related substances of Suftalan zinc by RP-HPLC is easy to operate and the results of the determination is accurate. |
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| Keywords: | Suftalan zinc content determination RP-HPLC |
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