脾气虚大鼠脾肝组织蛋白激酶C活性变化的实验研究

目的 :探讨脾气虚脾肝组织蛋白激酶C活性变化 ,以揭示脾气虚与脾肝组织PKC活性变化的关系。方法 :采用复合因素塑造大白鼠脾气虚证模型 ,采用底物磷酸化法检测模型脾肝组织蛋白激酶C活性。结果 :脾气虚证大鼠模型脾组织细胞膜中PKC活性明显降低 (P <0 0 0 1) ,而脾组织细胞浆中PKC活性无明显变化 (P >0 0 5 ) ;脾气虚证大鼠模型肝组织细胞膜PKC活性无明显变化 (P >0 0 5 ) ,肝组织细胞浆中PKC活性明显升高 (P<0 0 1)。健脾益气中药有提高脾组织细胞膜中PKC活性的趋势 ,但作用不显著 ,无统计学意义 ,而该中药能够降低脾组织细胞浆中PKC活性 (0 0 1

Experimental Study on Activities of Protein Kinase C in Spleen and Liver Tissues of Rats with Spleen-Qi Deficiency Syndrome

Objective:Research for relationship between spleen-Qi deficiency and activities of protein kinase C in spleen and liver tissues of rats.Method:Rat model with spleen-Qi deficiency syndrome were built by classical method and activities of protein kinase C were detected with substrate phosphorylation method.Results:PKC activities in cell membrane of spleen tissue of rats with spleen Yang deficiency syndromes decreased(P<0.01).PKC activities in cytoplasm of liver of rat with spleen Qi deficiency syndromes increased(P<0.01).Recipe for replenishing spleen Qi significantly decreased PKC activities in cell membrane of liver and in cytoplasm of spleen and liver (P<0.01).Conclusions:The changes of PKC activities are closely related to the pathological changes of spleen Qi deficiency.Chinese herbs for replenishing spleen Qi can adjust PKC activities in spleen and liver of rat with spleen yang deficiency syndrome.