猕猴白内障晶体上皮细胞超微结构研究

目的 探讨猕猴白内障晶体上皮细胞的超微结构,为将猕猴白内障作为人类白内障理想的动物模型提供病理学研究资料。方法 采用透射电镜对猕猴正常眼和猕猴白内障眼的晶体上皮细胞超微结构进行观察。结果 猕猴白内障眼晶体上皮细胞发生明显的变化：线粒体肿胀、空化、嵴消失,甚至形成凹空细胞;细胞水肿,细胞质溶解,甚至细胞崩解破坏;细胞核固缩、畸形,核膜间隙与核孙消失,核内异染色质浓集、周边化。结论 猕猴白内障上皮细胞

A study on lens epithelial cell ultrastructure in rhesus monkey cataract

OBJECTIVE: To investigate the ultrastructure of lens epithelial cells of normal eyes and cataractous eyes in rhesus monkeys. METHODS: Anterior capsules were taken from 4 normal lenses and 4 cataractous lenses of rhesus monkey eyes, and the samples were observed under transmission electron microscope. RESULTS: The ultrastructure of the cataractous lens epithelial cells changed obviously. Mitochondria with disappearance of crista were swollen, and vacuolar. Hollow cell with a single large vacuole in the cytoplasm could be seen. The cytoplasm presented dissolution. Cells were swollen and damaged, even cell membrane disruption could be seen. Karyopyknosis, nuclear deformity, disappearance of perinuclear cistern and nuclear pores could be found. The heterochromatin was concentrated and aggregated at the nuclear periphery. CONCLUSIONS: The ultrastructural changes of the lens epithelial cells in monkey cataractous lens are similar to that in human cataractous lens. Rhesus monkey cataract may be a suitable experimental model to investigate the formation of human cataract.