非抗病毒治疗相关的慢性乙型肝炎患者YMDD变异研究

目的：了解未经抗病毒治疗的慢性乙型肝炎患乙型肝炎病毒基因YMDD变异及其与前C区变异、HBV基因型的相关性，HBV DNA水平与变异发生的关系。方法：从126例均未接受拉米夫定治疗和近一年内未经任何抗病毒治疗的慢性乙肝患随机抽取25例为研究对象。血清YMDD变异检测运用微板核酸杂交—核酸定量法，HBV基因型采用PCR微板核酸杂交—ELISA技术，HBVDNA定量采用荧光定量PCR分析系统，HBV—M采用ELISA法。结果：7例存在YMDD变异，变异HBeAg全部阳性，不同的HBV基因型形式存在不同的YMDD变异率，HBVDNA水平与YMDD变异的发生不呈正相关。结论：HBV存在YMDD野生变异株，并非完全由抗病毒治疗引起，变异株与前C区变异和HBV基因型的相关性以及HBV DNA水平和变异发生的关系有待进一步研究。

Study of YMDD mutations in the patients of chronic hepatitis B untreated with Killing virus's medicines

Objective:To explore the relation between the YMDD mutations and it's pre C domain mutation and genotypes of hepatitis B virus(HBV),also to investigate the relationship of level of HBV DNA and possibility of mutations in the patients of chronic hepatitis B untreated with Killing virus's medicine Methods:The twenty five cases optional by 126 patients of chronic hepatitis B untreated with lamivudine and Killing virus's medicine one year were detected.The YMDD mutations of serum was detected by microplate sandwich hybridization nucleic acid ration,genotypes of HBV was detected by PCR microplate sandwich hybridization ELISA,HBV DNA quantifying assessment was detected by fluorescence ration PCR analysis,hepatitis B virus markers were detected by ELISA Results:Mutations were detected in seven of 25 sample,all mutation's HBeAg were positive. Mutations rate is not same in each genotype Conclusion:There are some YMDD mutations in the patients of chronic hepatitis B untreated with Killing virus's medicine.It need to farther research about the relationship of YMDD mutations and pre C domain mutation and genotypes of HBV