贲门癌原发性病灶和淋巴结转移病灶 P53 和 nm23 的变化

应用免疫组织化学方法对河南省食管癌高发区林州市居民贲门癌原发病灶和淋巴结转移病灶肿瘤抑制基因Ｐ５３和肿瘤转移抑制基因ｎｍ２３的变化进行研究。结果：在３７例贲门腺癌中，１８例原发病灶和转移病灶配对组织中一致性Ｐ５３免疫阳性反应的发生率为６７％（１２／１８）；定量分析表明，原发病灶中Ｐ５３蛋白聚集阳性细胞明显高于淋巴结转移病灶中Ｐ５３的表达；Ｐ５３免疫阳性的贲门腺癌淋巴结转移率（７８％）明显高于Ｐ５３免疫阴性的贲门腺癌淋巴结转移率（２９％，Ｐ＜０．０５）。３７例贲门癌组织中，ｎｍ２３免疫阴性者为２４例，占６５％，ｎｍ２３阴性者淋巴结转移率（７５％）明显高于ｎｍ２３表达阳性者淋巴结转移率（３１％，Ｐ＜０．０５）。在１０例贲门癌淋巴结转移组织中，淋巴结周边部转移病灶Ｐ５３阳性细胞数明显高于中心部。提示：Ｐ５３和ｎｍ２３在贲门癌转移过程中起一定作用。

Changes of P53 and nm23 in primary gastric cardia adenocarcinoma and lymph node metastatic lesions

The changes of tumor suppressor gene P53 and tumor metastatic suppressor gene nm23 in primary gastric cardia adenocarcinoma and lymph node metastatic lesions were determined from the patients in the high incidence area of esophageal cancer in Henan with immunohisto chemical methods.Results:Among the 37 cases of gastric cardia adenocarcinoma,18 cases showed consistant P53 positive immunoreactivity in the primary and lymph node metastatic cancer lesions(67%,12/28).Quantitative analysis showed that the number of P53 protein accumulation positive cells in the primary lesion was higher than that in lymph node metastatic cancer lesions.The metastasis rate in gastric cardia adenocarcinoma with P53 positive immunostaining was higher (78%) than that in negative P53 immunostaining (29%)(P<0.05).Out of the 37 cases of gastric cardia adenocarcinoma,24 cases showed negative immunostaining for nm23(65%).The metastatic rate in gastric cardia cancer with negative nm23 immunostaining (75%) was higher than that in nm23 positive immunostaining (31%)(P<0.05).At the same time,in 10 cases with lymph node metastatic lesions,the number of P53 positive cells in the surrounding area cancer nest was higher than that in the cancer nest center part.The present results suggest that P53 and nm23 may play an important role in the gastric cardia cancer metastasis.