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紫外分光光度法测定澳泰乐颗粒中总黄酮的含量
引用本文:许文学,邢学锋.紫外分光光度法测定澳泰乐颗粒中总黄酮的含量[J].中国中医药现代远程教育,2012,10(15):163-164.
作者姓名:许文学  邢学锋
作者单位:1. 南方医科大学中医药学院南方医科大学附属中西医结合医院,广州,510515
2. 南方医科大学中医药学院
摘    要:目的 建立紫外法测定澳泰乐颗粒中总黄酮的含量测定方法.方法 采用紫外分光光度计,检测波长510nm,以芦丁为标准品测定本品总黄酮含量.结果 测得线性回归方程为Y=2.1196X+0.0017,R2= 0.99969(n=5),平均回收率为98.75%,RSD为2.08%(n =9).结论 本方法简便,准确、重复性好.

关 键 词:澳泰乐颗粒  总黄酮  紫外分光光度法

Determination of Total Flavone in Aotaile Granules by UV
Xu Wenxue , Xing Xuefeng.Determination of Total Flavone in Aotaile Granules by UV[J].Chinese Medicine Modern Distance Education of China,2012,10(15):163-164.
Authors:Xu Wenxue  Xing Xuefeng
Institution:1*, (1 College of Traditional Chinese Medicine,Southern Medical University, Guangzhou 510515; 2 Integrative Medicine Hospital, Southern Medical University, Guangzhou 510515)
Abstract:Objective To establish a method for determination of total flavone in Aotaile granules by ultraviolet spectrophotometric method. Methods The content of total flavone was determined at 510 nm by ultraviolet spectrophotometric method with rutin as standard. Results The regression equation is Y= 2.11960X+0.0017, R2= 0.99969(n=5), the average recovery rate and RSD were 99.67% and 0.58%, respectively. Conclusion The method is simple, accurate and reproducible.
Keywords:Aotaile granules  Total flavone  Ultraviolet spectrophotometric method
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