柴胡不同炮制品对虚热证大鼠的干预作用

目的:综合评价柴胡的不同炮制品对于热性中药致虚热大鼠的干预作用。方法:SD健康雄性大鼠46只,随机分为正常组(6只),模型组(40只),模型组采用灌胃热性中药附子、干姜、肉桂(20 g·kg~(-1))方法建立虚热动物模型,成功造模后,随机将其分为正常组,模型组,知柏地黄丸组(知柏地黄丸,0.5 g·kg~(-1)),生品柴胡组,鳖血炒柴胡组,鳖血润柴胡组,酒柴胡组,醋柴胡组。各炮制品给药量均为3.15 g·kg~(-1),正常组灌以等量的双蒸水,治疗11 d,每日1次,观测大鼠的一般情况及代谢笼指标,通过测定并比较血清中D-木糖,血清三碘甲状腺原酸(T3),甲状腺素(T4),血浆环磷酸腺苷(cAMP),环磷酸鸟苷(cGMP)含量,乳酸(LD),乳酸脱氢酶(LDH),三磷酸腺苷(ATP)酶活力等的含量及大鼠各脏器指数。结果:与正常组比较,模型组大鼠体质量增长缓慢,肛温、饮水量、尿液量、体液消耗量增高,饮食量,D-木糖含量下降,血浆cAMP含量,cGMP含量,血清LD含量,LDH活力,肝组织中Na~+-K~+-ATP酶活力,Ca~(2+)-Mg~(2+)-ATP酶活力,血清T3和T4含量均上升(P0.05),说明成功复制虚热模型;与模型组比较,柴胡不同炮制品及知柏地黄丸组的D-木糖均上升(P0.05),血浆cAMP含量和cGMP含量均降低(P0.05),LD和LDH含量均上升(P0.05),各组中的ATP,T3,T4含量均降低(P0.05)。结论:柴胡的不同炮制品对于热性中药致虚热大鼠的各个生理指标均有趋于正常水平回转的趋势,对于大鼠的虚热证具有治疗作用,而不同的炮制品对于各个生理指标的影响效果不同,由此可见,不同的炮制品对于虚热大鼠具有不同程度地干预作用。

Effect of Different Processed Products of Bupleuri Radix on Deficiency Heat Syndrome Rats

Objective: To evaluate the intervention effect of different processed products of Bupleuri Radix on heat-induced deficiency heat syndrome rats. Method: Totally 46 healthy male rats were randomly divided into two groups:normal group (6 rats), and model group (40 rats). The model group was intragastrically administered with intragastrically Aconiti Lateralis Radix Praeparata, Zingiberis Rhizoma and Cinnamomi Cortex (20 g·kg^-1) to establish the internal heat model. After the successful modeling, they were randomly divided into normal group, model group, positive group (Zhibai Dihuangwan, 0.5 g·kg^-1), raw Bupleuri Radix group, Turtle blood Bupleuri Radix group, turtle blood combined with Bupleuri Radix group, wine Bupleuri Radix group, and vinegar Bupleuri Radix group. The dosage of each processed product was 3.15 g·kg^-1. In the normal group, rats were administered with the same amount of double-distilled water once a day for 11 days. The general conditions and metabolic cage index of rats were measured, and the radix bupleuri basal metabolic changes, D-xylose in serum, triiodothy ronine (T3), thy roxine (T4), cAMP, cGMP content, cortisol, lactic acid (LD), lactate dehydrogenase (LDH), activity of adenosine triphosphate(ATP) enzyme content and organ indexes of rats were compared. Result: Compared with the normal group, the body weight of rats in the model group increased slowly, the rectal temperature, water intake, urine volume and body fluid consumption elevated, and food intake and D-xylose content decreased; cAMP, cGMP content, LD in serum, LDH activity, adenosine triphosphate activity, T3 and T4 content increased (P<0.05). This indicated that the internal heat model was successfully established (P<0.05). Compared with the model group, the D-xylose in different processed product groups and positive drug group increased (P<0.05); the levels of cAMP and cGMP decreased (P<0.05); the levels of lactate and lactate dehydrogenase increase and the levels of ATP, T3, T4 in each group decreased (P<0.05). Conclusion: Bupleuri Radix''s different processed products can make the physiological indicators of the internal heat induced by traditional Chinese medicine in rats to return to normal, indicating a therapeutic effect on the internal heat syndrome in rats. However, the effects of different processed products vary on different physiological indexes. Therefore, different processed products have different degrees of intervention on the internal heat of rats.