巴西苏木红素与血清白蛋白及血浆成分结合率研究

 目的 建立测定巴西苏木红素与血浆结合率的平衡透析方法，并测定其体外与血浆总物质及牛血清白蛋白结合率。方法 采用平衡透析法测定巴西苏木红素与血浆总物质及牛血清白蛋白结合率，采用HPLC测定各成分的血药浓度及游离药物浓度，生物样品用乙酸乙酯液萃取的方法。 结果 在低、中、高3种浓度下，巴西苏木红素与牛血清白蛋白的结合率为38.5%~50.8%，与大鼠血浆总物质的结合率为86.2%~97.3%。结论 巴西苏木红素与大鼠血浆有较高的结合率，血浆中的磷脂部分可能为参与两者之间结合的主要物质。

Study on the Plasma Binding Rate of Brazilein

OBJECTIVE To establish an in vitro equilibrium dialysis method for studying the plasma binding rate of brazilein.METHODS Equilibrium dialysis was used to determine the binding rates of brazilein to bovine serum albumin and rat plasma,and the compound concentration was determined by HPLC.All the biological samples were handled with acetic ether-extraction.RESULTS The bovine serum albumin binding rates of brazilein at low,medium and high concentrations ranged from 38.5% to 50.8%,and the rat plasma binding rate of brazilein ranged from 86.2% to 97.3%.CONCLUSION Brazilein has high binding capacity to plasma,and the phospholipid components may have major contribution to the binding between brazilein and plasma.