| HPLC-ELSD法测定正脑颗粒中黄芪甲苷的含量 |
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| 引用本文： | 陆鹏,张典瑞,王玲.HPLC-ELSD法测定正脑颗粒中黄芪甲苷的含量[J].内蒙古中医药,2009,28(2). |
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| 作者姓名： | 陆鹏 张典瑞 王玲 |
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| 作者单位： | 1. 山东大学药学院,250012
2. 青岛国风药业股份有限公司,266510 |
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| 摘 要： | 目的:建立用高效液相色谱法测定正脑颗粒黄芪甲苷的方法。方法:采用高效液相色谱法,色谱柱为ZORBAXSB-C18(4.6mm×150mm,5μm),蒸发光散射检测器(Alltech2000ES);流动相为乙腈-水(32:68);柱温为30℃。结果:黄芪甲苷在1.3968～9.7776μg/ml之间呈良好的线性关系(r=0.9994),平均回收率为97.5%,RSD=1.23%。结论:本方法简便可行,精密度高,重现性好,可用于正脑颗粒的质量控制。
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| 关 键 词： | HPLC-ELSD 正脑颗粒 黄芪甲苷 含量 |
Determination of Astragaloside IV in Zhengnao Granules by HPLC-ELSD |
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| LU Pen,ZHANG Dian-rui,WANG Ling.Determination of Astragaloside IV in Zhengnao Granules by HPLC-ELSD[J].Nei Mongol Journal of Traditional Chinese Medicine,2009,28(2). |
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| Authors: | LU Pen ZHANG Dian-rui WANG Ling |
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| Abstract: | Objective:To establish the methods of determining astragaloside Ⅳin Zhengnao granules by HPLC.Methods:HPLC-ELSD was performed on ZORBAX SB-C18 Column(5μm, 4.6 mm × 150 mm), acetonitrile - wate(r32 : 68)served as mobile phase , col-umn temperature was 30 ℃.Results:There was good linear relationship between the concentrations and the peak-areas of astragalo-side Ⅳ(1.3968～9.7776μg/ml, r=0.9994 ).The average recovery was 97.5%, RSD=1.23%( n = 6).Conclusion:The methods established are simple, feasible, accurate,... |
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| Keywords: | HPLC-ELSD |
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