长毛风毛菊中总香豆素的提取工艺及抗氧化活性研究

目的：研究长毛风毛菊中总香豆素的回流提取工艺和体外抗氧化活性。方法：以乙醇浓度、料液比、提取时间、提取次数为考察因素，以总香豆素的提取率为指标进行正交试验，优选长毛风毛菊最佳提取工艺，同时测定了各萃取部位对DPPH自由基的清除能力。结果：长毛风毛菊总香豆素最佳提取工艺即乙醇浓度40%、料液比1∶50、提取5次，每次1 h。长毛风毛菊提取物各萃取部位对DPPH自由基有一定的清除能力，最大清除率依次为乙酸乙酯萃取物> 正丁醇萃取物> 氯仿萃取物> 水残留物> 石油醚萃取物。结论：本研究建立的长毛风毛菊中总香豆素提取工艺简单高效，乙酸乙酯和正丁醇部位具有一定的抗氧化作用，可为长毛风毛菊中总香豆素的资源开发和工业化提取提供参考。

Research on Extraction and Antioxidant Activity of Total Coumarin from Saussurea Hieracioides

Objective: To study the reflux extraction process and in vitro antioxidant activity of total coumarin in Saussurea Hieracioides. Methods: Taking the concentration of ethanol, the ratio of material and liquid, the extraction timeand the extraction times as the factors, and taking the extraction rate of total coumarin as the index to conduct orthogonaltest, the best extraction process of S. hieracioides was optimized. Meanwhile, the free radical scavenging capacity ofDPPH was measured at each extraction part. Results: The optimal extraction process of total coumarin of S. hieracioides was ethanol concentration of 40%, solid-liquid ratio of 1:50, extraction for 5 times and each time for 1h. All parts for theextraction of S. hieracioides have certain scavenging capacity for DPPH free radical. And the maximum clearance rate:ethyl acetate extract > n-butanol extract > chloroform extract > water residue > petroleum ether extract. Conclusion: Theextraction process of total coumarin from S. hieracioides in this study is simple and efficient. The ethyl acetate and nbutanolfractions have certain antioxidation effects, which can provide references for the resource development andindustrial extraction of total coumarin in S. hieracioide.