HPLC法同时测定肠肽胶囊中三七皂苷R1、人参皂苷Rg1及Rb1含量

目的:建立肠肽胶囊中三七有效成分的HPLC含量测定方法。方法:色谱柱为SinoChrom ODS-BP（150 mm×4.6mm,5μm）,以乙腈（A）和水（B）为流动相,梯度洗脱,检测波长为203 nm,流速为1.0 ml·min^-1,柱温为室温,进样量为20μl。结果:三七皂苷R₁、人参皂苷Rg₁及人参皂苷Rb₁的线性范围依次为63.9～319.5μg·ml^-1、112.6～563.2μg·ml^-1、102.5～512.8μg·ml^-1,线性关系良好（r值分别为0.999 7、0.999 4、0.999 9）;三种皂苷的平均回收率分别为96.47%、98.75%,97.55%,RSD分别为1.52%、1.73%、1.81%（n=6）。结论:本方法简便可行、准确、灵敏度高、专属性强,可用于肠肽胶囊的质量控制。

Simultaneous Determination of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 in Changtai Capsules by HPLC

Objective： To develop an HPLC method for the simultaneous determination of active ingredients in Changtai capsules. Method： The HPLC method was carried out on a SinoChrom ODS-BP column （ 150 mm × 4. 6 mm,5 μm） at room temperature. The mobile phase consisted of acetonitrile （A）-water （B） with gradient elution, the flow rate was 1.0 ml·min^-1, the UV detection wavelength was at 203 nm and the injection volume was 20 μl. Result： The calibration curves showed good linear correlation within the range of 63.9-319. 5 μg·ml^-1 for Notoginsenoside R1 （r =0. 999 7） ,112. 6-563.2 μg·ml^-1 for Ginsenoside Rg1 （r =0. 999 4） and 102. 5-512. 8 μg· ml^-1 for Ginsenoside Rb1 （r = 0. 999 9）. The average recovery of Notoginsenoside Rl , Ginsenoside Rg1 and Ginsenoside Rbl was 96. 47% with RSD of 1.52% ,98.75% with RSD of 1.73% and 97.55% with RSD of 1.81% ,respectively. Conclu sion： The method is simple, feasible,precise, sensitive and strongly specific, and can be used for the quality control of Changtai capsules.