嗜铬细胞瘤超微结构和P21,P53的过量表达

目的：研究嗜铬细胞瘤的超微结构和ｒａｓＰ２１、Ｐ５３过量表达的情况。方法：利用透射电镜和流式细胞术方法进行。结果：１４例瘤细胞内线粒体发达，粗面内质网丰富，胞浆内充满大量致密颗粒，超微结构特征与临床表现方面没有特征性差异；１０例进行流式细胞术分析表明异倍体肿瘤５０％，ｒａｓＰ２１表达阳性７例，Ｐ５３表达阳性５例，两者均表达阳性４例，进一步分析发现，Ｐ２１表达阳性者，其细胞增殖指数明显高于Ｐ２１表达

FINE STRUCTURE,PHOIDY AND OVEREXPRESSION OF RAS P21 AND P53 IN PHEOCHROMOCYTOMA

Objective:To study fine structure of tumor,ploidy and overexpression of ras P 21 and P 53 and clinical finding correlated with pheochromocytoma.Methods:Electron micrographic analysis in tumor morpho1ogy and DNA ploidy,and P 21 and P 53 assessed by flow cystometry in tumor cells.Results:The study showed that no correlation was found between the clinical symptomato1ogy and the fine structure,and no morphologic evidence was found to substantiate the existence of functionally different types of pheochromocytoma;half of the tumors have aneupliod stem cell lines;there were overexpression of ras P 21 in 7 cases and P 53 in 5 cases,with both positive expression in 4 cases,and higher product index(PI) in the tumor with ras P 21 positive expression than that P 21 negative (P<005).Conclusion:The fine structure in pheochromocytoma is very simile with the endocrine cells and there are the overexpression of P 21 and P 53 in this tumor.