锰超氧化物歧化酶9Ala／Va1基因多态性与冠心病的关系

目的:探讨锰超氧化物歧化酶9 Ala/Val(MnSOD9 Ala/Val)基因多态性与冠心病、血浆总超氧化物歧化酶(T-SOD)和锰超氧化物歧化酶(MnSOD)活性的关系.方法:采用ABI 3100 DNA测序仪检测147例冠心病患者和108例对照组的MnSOD9 Ala/Val基因多态性的基因型,采用比色法测定血浆T-SOD和MnSOD活性.结果:与对照组比较,冠心病患者的血浆T-SOD和MnSOD活性明显降低,C反应蛋白(CRP)和颈动脉内一中膜厚度(IMT)则显著增高;冠心病患者的MnSOD VV基因型和V等位基因携带者较对照组明显增多;MnSOD VV基因型的T-SOD和MnSOD活性较AA基因型明显降低,并凡MnSOD 9V等位基因与T-SOD活力、MnSOD活力和高密度脂蛋白呈负相关;与空腹血糖、胆因醇、甘油三酯、低密度脂蛋白、收缩压、IMT和CRP呈正相关.结论:冠心病患者的抗氧化能力明显降低,因而炎症反应和动脉硬化程度加重;MnSOD 9 Ala/Val基因多态性通过影响血浆MnSOD活性和冠心病的危险冈素而参与冠心病的发病.

Association Between Manganese Superoxide Dismutase 9Ala/Val Genetic Polymorphisms and Coronary Heart Disease

Objective: To study the associations between manganese superoxide dismutase 9Ala/Val(MnSOD 9 Ala/Val) genetic polymorphisms and coronary heart disease(CHD).Methods: The ABI 3100 DNA sequencer was applied to identify the genotype of MnSOD 9 Ala/Val genetic polymorphisms,and colorimeter was used to detect plasma activities of total superoxide dismutase(T-SOD) and MnSOD in 147 CHD patients and 108 controls.Results: Compared with that of controls,the plasma activity of T-SOD and MnSOD reduced obviously,while C-reaction protein(CRP) concentration and intima-medium thickness(IMT) in carotid artery increased markedly in CHD patients.There were more VV genotype and V allele of MnSOD 9 Ala/Val genetic polymorphisms in CHD patients than in controls.The activity of plasma T-SOD and MnSOD decreased,while CRP and IMT increased in individuals with MnSOD 9 VV genotype compared in those with AA genotype,and MnSOD 9V allele correlated negatively with the activity of T-SOD,MnSOD and serum HDL level,positively with fasting plasma glucose,total cholesterol,triglyceride,LDL,systolic blood pressure,IMT and CRP.Conclusion: The antioxidative ability decreased,while inflammatory reaction and degree of atherosclerosis increased in CHD patients.Gene polymorphisms of MnSOD 9 Ala/Val involves in the pathogenesis of CHD by influencing the plasma activities of T-SOD,MnSOD and risk factors of CHD.