脂联素基因+45T/G单核苷酸多态性与汉族男性早发冠心病的相关性

目的 探讨脂联素基因+ 45T/G单核苷酸多态性与汉族男性早发冠心病的相关性.方法 将423例汉族男性患者(＜ 55岁)冠状动脉造影结果分为冠心病组(358例)及对照组(65例),采用PCR-RFLP分析脂联索基因+45T/G单核苷酸多态性并检测脂联素及血脂、血糖等相关临床指标.结果 冠心病组TG/GG基因型比例为51.1％,对照组TG/GG基因型比例为35.4％,差异有统计学意义(x2 =5.45,P=0.022).矫正其他冠心病易感因素后,Logistic回归发现+45T/G单核苷酸多态性与汉族男性早发冠心病发生相关(OR=1.843,P=0.035,95％CI:1.045～3.250).结论 脂联素基因+ 45T/G单核苷酸多态性与汉族男性早发冠心病发生相关,TG/GG基因型可能为汉族男性早发冠心病易感基因型.

Correlation of adiponectin gene SNP +45T/G polymorphism and premature coronary heart disease of Han ethnic males

Objective To examine the relationship between single nucleotide polymorphism (SNP) of adiponectin (APN) locus +45T/G and Han male patients with premature coronary artery heart disease (pCAD).Methods A total of 423 male patients of Han ethnic group ( ＜55 yr old) undergoing coronary arteriography were recruited.Among them,358 patients were diagnosed as pCAD while another 65 normal control (NC).All subjects were genotyped for adiponectin gene SNP + 45 by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) assay.Results Statistical differences of TG/GG genotype ratio were observed between two groups.The TG/GG genotype ratio in the pCAD group was 51.1％ versus 35.4％ in the NC group ( x2 =5.45,P =0.022).After an adjustment of conventional risk factors,such as age,body mass index (BMI) and hypertension,the adiponectin gene SNP + 45 TG/GG genotype was strongly associated with Han ethnic group male pCAD by binary logistic regression analysis ( OR =1.843,P =0.035,95％ CI:1.045 ～3.250).And there were statistical differences between TT genotype and TG/GG genotype among pCAD subjects in the following factors:BMI,total cholesterol (TC),low-density lipoprotein-C (LDL-C) and APN level (P ＜0.05).By Pearson correlation analysis,APN was negatively correlated with TC,LDL-C and SNP + 45T/G polymorphism. Conclusion The SNP of adiponectin locus +45T/G is associated with male pCAD.And TG/GG genotype is a possible predisposing gene for Han ethnic group male pCAD.