| 心肌细胞培养方法和活细胞噻唑兰法测定 |
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| 引用本文： | 王达理,曾爱萍,南柏松.心肌细胞培养方法和活细胞噻唑兰法测定[J].吉林医药学院学报,1999(4). |
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| 作者姓名： | 王达理 曾爱萍 南柏松 |
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| 作者单位： | 解放军第454医院!南京市210002(王达理，曾爱萍)，第四军医大学唐都医院!西安市710038(南柏松) |
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| 摘 要： | 目的:介绍心肌细胞培养方法和研究比色分析法测定活心肌细胞.方法:接种不同数量细胞培养后行噻唑兰(MTT)比色分析;正常培养、含不同浓度维拉帕米培养的细胞行MTT代谢比色分析,并测乳酸脱氢酶(LDH)活性.结果:比色值与接种细胞数量、细胞释放的LDH活性呈高度相关(r=0.996、-0.986,P<0.01).结论:MTT比色分析法可用于测定活心肌细胞.
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| 关 键 词： | 噻唑兰比色分析法 维拉帕米 心肌细胞 乳酸脱氢酶 |
Cultivation and MTT assay of living myocardiac cells |
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| Wang Dali,Zeng Aiping,Nan Baisong.Cultivation and MTT assay of living myocardiac cells[J].Journal of Jilin Medical College,1999(4). |
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| Authors: | Wang Dali Zeng Aiping Nan Baisong |
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| Institution: | Wang Dali,Zeng Aiping,Nan Baisong The 454th Hospital of PLA,Nanjing,210002 |
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| Abstract: | ve: To introduce the cultivation of myocardiac cells and study how to use colorimetric analysis in test of living myocardiac cells. Method: Different densities of cells were seeded and cultivated for MTT assay. Afterward, the cells, normally cultivated or cultivated in different concentrations of verapamil, were tested for the activity of lactic dehydrogenase. Result: MTT assay gave response curves which were highly correlated with the seeding densities (r = 0.996,P<0.01) and LDH activity (r= - 0.986, P < 0.01) .Conclusion: MTT assay can be used in test of living myocardiac cells. |
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| Keywords: | MTT colorimetric assay verapamil myocardiac cell lactic dehydrogenase |
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