黄疣海参皂苷 hillaside A 和 hillaside B 的体外抗真菌及抗肿瘤活性

目的阐明黄疣海参hillasideA和hillasideB的抗肿瘤及体外抗真菌活性。方法对黄疣海参的总提取物采取硅胶柱色谱及半制备高效液相色谱（HPLC）法等分离手段进行活性成分追踪分离，通过理化性质及波谱学手段进行化学结构鉴定，以磺酰罗丹明B蛋白染色（SRB）法评价化合物的抗肿瘤活性。结果分离得到了2个新的三萜皂苷元类化合物，命名为hillasideA和hillasideB。2个新化合物均显示了较强的体外抗真菌及抗肿瘤活性。结论研究为研制新的抗肿瘤药物提供了先导化合物，为充分开发利用我国的海洋生物资源提供了一定的物质基础和科学依据。

Study on In Vitro Antifungai and Antitumor Activities of Hillaside A and Hillaside B from Sea Cucumber Holothuria nobilis

Objective To illuminate the in vitro antifungai and antitumor activities of hillaside A and hillaside B from sea cucumber Holothuria nobilis. Methods The total extract from sea cucumber Holothuria nobilis was performed the trace separation of the active ingredients by adopting the silica gel column chromatography and the semipreparative HPLC. Their chemical structures were identified by the physicochemical properties and spectroscopic methods. Their antitumor activities were evaluated by the SRB method. Results Two new triterpene glycosides were obtained and their structures were identified, named as hillaside A and hillaside B. Two new saponins showed the strong in vitro antifungai and antitumor activities. Conclusion This research provides the lead compounds for studying the new antitumor drugs and a certain material basis for full exploitation and utilization of the marine biological resources in our country.