海洋真菌的分离、抗肿瘤活性筛选与发酵条件研究

目的 对青岛海域海水、海泥样品进行真菌选择性分离培养，并对其发酵物进行抗肿瘤活性筛选及活性菌株发酵条件的研究。方法 采用温敏型小鼠乳腺癌tsFT210细胞系，运用流式细胞术结合显微镜镜检，以细胞周期抑制和细胞凋亡诱导为活性筛选指标．并通过该活性指标对培养基、发酵条件及提取分离条件进行考查。结果 添加青霉素、链霉素构成的土豆培养基具有很好的培养真菌选择性；共分离得到真菌207株，活性筛选得到阳性菌19株，其中编号Z8 3200的真菌具有显著的细胞凋亡活性。

Study on the isolation of marine fungi, screening of their antitumor activity and fermentation conditions

Objective To isolate fungi from sea sediment and sea water samples collected from the neritic environment in Qingdao, screen their antitumor activity and study on their fermentation condition. Methods The antitumor activity was assayed by flow cytometry in mouse tsFT210 cells. Results The medium added penicillin and streptomycin possesses good selectivity. 207 strains of fungi were isolated and among them, 19 strains showed antitumor activity, and one fungus(Z 83200)pocesses strong cell apoptosis inducing activity.