| 高效液相色谱法测定川贝清肺膏中甘草酸的含量 |
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| 引用本文： | 曾丽,薛非凡,王丽玉,李炳森.高效液相色谱法测定川贝清肺膏中甘草酸的含量[J].中国医院用药评价与分析,2013(7):633-635. |
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| 作者姓名： | 曾丽 薛非凡 王丽玉 李炳森 |
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| 作者单位： | [1]湖北省襄阳市第五人民医院,湖北襄阳441000 [2]湖北省襄阳市食品药品监督管理局,湖北襄阳441021 [3]珠海正太制药有限公司,广东珠海519110 |
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| 摘 要： | 目的：建立高效液相色谱法（HPLC）测定川贝清肺膏中甘草酸的含量。方法：采用Kromasil-C18（4.6 mm×150 mm,5μm）为色谱柱;流动相：乙腈-水-冰醋酸（37∶63∶2）;检测波长：250 nm;流速：1 ml/min;柱温为室温（25℃）。结果：样品中甘草酸得到很好的分离,当甘草酸在10.0～104.0μg范围内呈线性关系（r=0.999 9,n=5）,平均加样回收率为100.99%,RSD为0.96%（n=6）。结论：本法简便、准确、可靠、专属性强、重现性好,可用于川贝清肺膏中甘草酸的含量测定。
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| 关 键 词： | 川贝清肺膏 甘草酸 高效液相色谱法 含量测定 |
Determination of Glycyrrhizic Acid in Chuanbeiqingfei Cream by HPLC |
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| ZENG Li,XUE Fei-fan,WANG Li-yu,LI Bing-sen.Determination of Glycyrrhizic Acid in Chuanbeiqingfei Cream by HPLC[J].Evaluation and Analysis of Drug-Use in Hospital of China,2013(7):633-635. |
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| Authors: | ZENG Li XUE Fei-fan WANG Li-yu LI Bing-sen |
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| Institution: | 3 ( 1. Hubei Xiangyang Fifth People＇ s Hospital, Hubei Xiangyang 441000, China; 2. Hubei Xiangyang Food and Drug Administration, Hubei Xiangyang 441021, China; 3. Zhuhai Zhengtai Pharmaceutical Co. Ltd. , Guangdong Zhuhai 519110, China) |
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| Abstract: | OBJECTIVE：To establish HPLC method for determination of glyeyrrhizic acid in Chuanbeiqingfei Cream. METHODS： The separation of samples was performed on Kromasil-Cls （4. 6 mm ×150 mm, 5μm） chromatographic column with mobile phase consisting of acetonitrile-water-glacial acetic acid （37： 63： 2） at detection wavelength of 250 nm, flow rate of 1.0 ml/min and column temperature of 25 ~E. RESULTS ： The glycyrrhizic acid in samples was well-separated. The optimal linear range of glycyrrhizic acid was 10. 0-104. 0 p~mL （ r = 0. 999 9, n = 5 ） with average recovery of 100. 99% （RSD =0. 96%, n = 6）. CONCLUSION： The method is simple, accurate, reliable, specific and reproducible, and it is applicable for content determination of glycyrrhizic acid in Chuanbeiqingfei Cream. |
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| Keywords: | Chuanbeiqingfei Cream Glycyrrhizic acid HPLC Determination |
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