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| SPA-ELISA检测棕果蝠血清冠状病毒抗体的研究 | |
| 引用本文： | 周杰,廖玉学,陈忠,李玉春,高璐璐,陈亿雄,蔡练功,陈清,俞守义.SPA-ELISA检测棕果蝠血清冠状病毒抗体的研究[J].南方医科大学学报,2008,28(5):736-738. |
| 作者姓名： | 周杰 廖玉学 陈忠 李玉春 高璐璐 陈亿雄 蔡练功 陈清 俞守义 |
| 作者单位： | 1. 南方医科大学公共卫生与热带医学学院流行病学系,广东,广州,510515 2. 海南师范大学生物科学系,海南海口,571158 3. 山东大学威海分校海洋学院,山东,威海,264209 |
| 基金项目： | 国家自然科学基金 , 广东省科技厅科技计划 |
| 摘 要： | 目的 建立一种敏感、简便的检测蝙蝠血清冠状病毒抗体的方法.方法 利用葡萄球菌A蛋白(SPA)能够与某些哺乳动物IgG抗体Fc段非特异性结合的特点,对市售人冠状病毒抗体ELISA试剂盒进行改造,建立SPA-ELISA法,使其适用于蝙蝠血清冠状病毒抗体的检测,并对采集到的55份棕果蝠血清冠状病毒抗体进行检测.结果 用SPA-ELISA方法对人冠状病毒试剂盒中阳性与阴性对照、SARS病人恢复期血清、空白对照的检测都得到理想的结果,并从55份棕果蝠血清中检测出2例阳性,阳性率为3.64％(2/55),中和试验进一步证实了结果的真实性.结论 所建立的方式适用于检测棕果蝠血清冠状病毒抗体. |
| 关 键 词： | SPA-ELISA 冠状病毒抗体 蝙蝠 检测 果蝠 恢复期血清 冠状 病毒 抗体 研究 bats fruit serum antibodies detecting method 中和试验 阳性率 空白对照 病人 SARS 阴性对照 结果 |
| 文章编号： | 1673-4254(2008)05-0736-03 |
| 修稿时间： | 2008年1月16日 |
Development of a SPA-ELISA method for detecting anti-coronavirus IgG antibodies in serum samples from fulvous fruit bats |
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| ZHOU Jie,LIAO Yu-xue,CHEN Zhong,LI Yu-chun,GAO Lu-lu,CHEN Yi-xiong,Cai Lian-gong,CHEN Qing,YU Shou-yi.Development of a SPA-ELISA method for detecting anti-coronavirus IgG antibodies in serum samples from fulvous fruit bats[J].Journal of Southern Medical University,2008,28(5):736-738. | |
| Authors: | ZHOU Jie LIAO Yu-xue CHEN Zhong LI Yu-chun GAO Lu-lu CHEN Yi-xiong Cai Lian-gong CHEN Qing YU Shou-yi |
| Institution: | Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. paburaw@hotmail.com |
| Abstract: | OBJECTIVE: To develop an simple and sensitive method for detecting anti-coronavirus IgG antibodies in bat sera based on enzyme-linked immunosorbent assay (ELISA). METHODS: A commercial ELISA kit for detecting SARS-CoV antibody was modified for detecting coronavirus antibodies in bat serum samples. The second antibody in the kit was replaced with horseradish peroxidase-conjugated protein-A (HRP-SPA) based on the characteristics of binding between Staphylococcus aureus protein A (SPA) and mammal IgG Fc fragment. The sera of 55 fulvous fruit bats (Rousettus dasymallus) were tested using the SPA-ELISA. RESULTS: The test results of the positive and negative controls in the kit and the serum samples from convalescent ;patient were consistent with expectation. Coronavirus antibody was detected in 2 out of the 55 bat serum samples. Serum neutralization test confirmed the validity of the SPA-ELISA method. CONCLUSION: This SPA-ELISA method is applicable for detecting coronavirus antibody in bat sera. |
| Keywords: | Staphylococcus aureus protein A enzyme-linked immunosorbent assay coronavirus antibody bats |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |