P27^kip1与出核因子CRM1在人淋巴瘤细胞系中的表达及相互关系

目的研究P27kip1与出核因子CRM1在淋巴瘤细胞中的表达及相互关系。方法运用血清饥饿同步化处理淋巴瘤细胞株Jurkat和Raji,10%血清刺激细胞增殖。Western blot分别检测生长阻滞及增殖的淋巴瘤细胞中P27kip1与CRM1的表达。用核浆分离方法检测P27kip1与CRM1分别在胞核、胞质的定位。免疫沉淀检测P27kip1与CRM1在淋巴瘤细胞中的结合。结果在不同状态的淋巴瘤细胞中,P27kip1与CRM1蛋白水平的表达均表现为负相关,并且P27kip1的核内外分布与CRM1的核内外分布一致。免疫沉淀结果验证在淋巴瘤细胞中P27kip1与CRM1相互结合。结论出核因子CRM1可能通过与P27kip1结合而影响P27kip1的核内外分布及表达水平,从而参与调控淋巴瘤细胞的生长。

Expression and relationship of P27~(kip1) with nuclear export factor CRM1 in human Lymphoma cell lines

Objective To investigate the expression and relationship of P27kip1 with nuclear export factor CRM1 in lymphoma cells. Methods Cells were synchronized by serum starvation, and 10%serum stimulating proliferation. The expression of P27kip1 and CRM1 was detected by cell fractionation and Western blot. Results The albumen amount of P27kip1 increased while albumen amount of CRM1 was decreased. The reverse changes happened after serum release. The location of P27kip1 was accorded with the expression of CRM1. P27kip1 and CRM1 could form compound in Jurkat and Raji cells as detected by immunoprecipitation. Conclusion CRM1 may influence the location and expression of P27kip1 through integrating with P27kip1, and then may participate in regulating the growth of lymphoma cells.