经皮注射骨形态发生蛋白和自体红骨髓修复兔骨缺损与骨不连

目的；评价骨形态发生蛋白（ＢＭＰ）与自体红骨髓复合经皮注射在修复骨缺损与骨不连中的作用。方法：２０只成年新西兰大白兔，双人则桡骨中上段截去１５ｍｍ，包括骨膜，每１０个缺损为一组，随机分为４组，分别植入骨形态发生蛋白（ＢＭＰ）／自体红骨髓（ＲＢＭ）／聚乙比咯烷酮（ＰＶＰ）－Ａ组，ＢＭＰ／ＰＶＰ－Ｂ组，ＲＢＭ／ＰＶＰ－Ｃ组和ＰＶＰ－Ｄ组，植入后２ｗｋ￣８ｗｋ做Ｘ线检查、组织学检查及生物力学试验，结果：

Reparation of bone defect and nonunion by percutaneous injection of bone morphogenetic protein and red bone marrow inrabbits

AIM: To evaluate the percutaneous injection ofbone morphogenetic protein (BMP ) and red bone marrow(RBM) in repairing bone defects and nonunion. METHODS:Twenty Newzeland rabbits aged I -- 1. 5 years were randomlydivided into four groups. A fifteen--millimeter radial bone defect was created based on the established model of bonenonunion in rabbits. One group was injected withpolyvinylpyrrolidone (PVP) as control (group D). The others were injected respectively with BMP/RBM/PVP (groupA), BMP/PVP (group B) and BM/PVP (group C ). Thebone repair was assessed by serial roentgenographic and histological studies and biomechanical tests from two to eightweeks after the injection. RESULTS: New bone was inducedin all groups except the control group. In group A, the newlyformed bone highly resembled the normal bone morphologically and histologically. In group D, the cut end of the radiusbecame ossified and the bone marrow canal was blocked. Thehistologic study revealed that seven of the eight defects ingroup A were united with massive bone formation and thebone marrow canal was recanalized eight weeks postoperatively. CONCLUSION: The results of this study suggest thatthe percutaneous injection of BMP/RBM/PVP has great potentials for clinical use in repairing bone defects andnonunion.