增生性玻璃体视网膜病变基质金属蛋白酶的定量研究

目的:研究增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)玻璃体中基质金属蛋白酶(matrix metalloproteinases,MMPs)的表达,探讨MMPs在PVR病理过程中的作用。方法:PVR患者采用标准三切口巩膜扁平部玻璃体切割术(pars plana vitrectomy,PPV),取未稀释的玻璃体21只眼,PPV术后复发的玻璃体腔液20只眼,意外死亡的正常人玻璃体10只眼,采用明胶酶谱分析法定量分析MMP-2和MMP-9活性水平。结果:PVR玻璃体有MMP-2活性水平增高,与正常玻璃体比较差异有显著性意义(P<0.05)。21眼PVR玻璃体中13只眼有MMP-9活性水平增高,平均(171.52±13.17)扫描单位。20眼PPV术后PVR复发的玻璃体腔液19只眼有MMP-9活性水平增高,平均(156.01±37.21)扫描单位。正常人玻璃体无MMP-9的表达。结论:PVR玻璃体有MMP-2和MMP-9活性水平增高,MMP-9活性水平增高可能与术后PVR复发有关。眼科学报2003;19:130-132。

A Quantitative Study of Matrix Metalloproteinases in Proliferative Vitreoretinopathy

PURPOSE: To investigate the presence of matrix metalloproteinases (MMPs) in vitreous of patients with proliferative vitreoretinopathy (PVR). To study MMPs involved in the pathogenesis of the PVR. METHODS: Twenty-one PVR vitreous samples were from patients who were performed pars plana vitrectomy (PPV). Twenty vitreous liquid samples were from patients with postoperative recurrent PVR after PPV. Ten vitreous samples were normal group. The activities of MMP-2 and MMP-9 were determined by quantitative gelatinase zymography. RESULTS: MMP-2 was detected in all of PVR and normal group. Vitreous samples were obtained, whereas MMP-9 was found in 13 of 21 vitreous with preoperative PVR, average (171.52 +/- 13.17)scanning units. MMP-9 was detected in 19 of 20 vitreous with postoperative recurrent PVR, average(156.01 +/- 37.21)scanning units. CONCLUSION: MMP-2 and MMP-9 were found in the vitreous of PVR. Elevated levels of MMP-9 may play a role in the development of postoperative PVR.