| 柱前衍生高效液相色谱法测定复方氨基酸注射液中氨基酸的含量 |
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| 作者姓名： | 邓红英 张永文 李永贵 |
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| 作者单位： | 山东大学齐鲁医院药品调剂科,山东 济南250012;济南市中心医院药剂科,山东 济南250014;山东省药学科学院,山东省化学药物重点实验室,山东 济南250101;山东省药学科学院,山东省医用高分子材料重点实验室,山东 济南250101 |
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| 基金项目： | 山东省重点研发计划（No.2018GSF118132） |
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| 摘 要： | 目的建立以2,4-二硝基氟苯为衍生化试剂,高效液相色谱法(HPLC)测定复方氨基酸注射液(18AA)中各氨基酸含量的方法。方法采用Agilent HC-C 18色谱柱,乙腈-水(60∶40)为流动相A,乙腈-磷酸盐缓冲液(9∶91)流动相B,梯度洗脱,流速1.0 mL·min-1,检测波长360 nm,柱温27℃。结果复方氨基酸注射液中各氨基酸分离度良好,线性相关系数均大于0.9989,平均回收率均在98%~102%之间,RSD%均小于2%。结论本方法可以用于复方氨基酸注射液(18AA)中氨基酸检测。
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| 关 键 词： | 柱前衍生 2 4-二硝基氟苯 复方氨基酸注射液 18AA 含量测定 |
Determination of amino acids in Compound Amino Acids Injection by HPLC after pre-column derivatization |
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| Authors: | DENG Hongying ZHANG Yongwen LI Yonggui |
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| Institution: | (Department of Pharmaceutical Dispensing,Qilu Hospital of Shandong University,Jinan 250012,China;Department of Pharmacy,Jinan Central Hospital,Jinan 250014,China;Shandong Provincial Key Laboratory of Chemical Drug,Shandong Academy of Pharmaceutical Sciences,Jinan 250101,China;Shandong Provincial Key Laboratory of Biomedical Polymers,Shandong Academy of Pharmaceutical Science,Jinan 250101,China) |
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| Abstract: | Objective To establish a method for the determination of amino acids in compound amino acid injection(18AA)by high performance liquid chromatography(HPLC)with 2,4-dinitrofluorobenzene as derivative reagent.Methods An Agilent HC-C 18 column was used in the gradient elution with acetonitrile-water(60∶40)as mobile phase A and acetonitrile-phosphate buffer(9∶91)as mobile phase B.The flow rate was 1.0 mL·min-1,the detection wavelength was 360 nm and the column temperature was 27℃.Results The separation degree of amino acids in compound amino acid injection was good.All the linear correlation coefficients were greater than 0.9989.The average recoveries were between 98%and 102%with RSD s were less than 2%.Conclusion This method can be used for the determination of amino acids in compound amino acid injection(18AA). |
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| Keywords: | Pre-column derivatization 2 4-dinitrofluorobenzene Compound Amino Acids Injection 18AA Determination |
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