不同炮制时间制首乌致泻作用量-时-效相关性变化及其药理数学模型化研究

目的:初步研究不同炮制时间何首乌致泻强度与化学含量相关性,并初步建立对其致泻药理数学模型。方法:采用小鼠泻下半数有效量(ED50)为评价指标,考察何首乌生品和经4个不同炮制时间处理过的何首乌制品致泻强度的差异;用高效液相色谱法测定生品及不同制品中蒽醌类化合物含量,揭示不同何首乌样品与致泻强度二者量-时-效关系,并建立不同炮制时间的何首乌致泻药效的数学模型。结果:不同炮制时间制首乌的ED50所需给药剂量分别为:2.562、4.085、11.146、14.692、21.068g/kg(生药/体重);致泻强度顺序为:生品何首乌>1h制品何首乌>2h制品何首乌>3h制品何首乌>4h制品何首乌;结合型蒽醌含量高低顺序为:生品何首乌>1h制品何首乌>2h制品何首乌>3h制品何首乌>4h制品何首乌。讨论:何首乌生品、经不同炮制时间处理后的制何首乌之间致泻强度存在较大差异;致泻强度与结合型蒽醌含量间具有量-时-效关系;将生物检测方法和数学模型化模式联系起来,更全面的保证中药质量及中医临床用药安全。

Research on Changes of Purgative Action-time-effect Relationship among Different Processing Time of Prepared Polygonum multiflorum and on Pharmacological Mathematic Model

Objective： A preliminary study on purgative strength among different processing time of Polygonum multiflorum and chemical content correlation, the initial establishment of the mathematical model of diarrhea induced by pharmacological. Methods： The mice purgative median effective dose （ ED50 ） as the evaluation index, Polygonum multiflorum thunb and study the differences of purgative intensity between raw medicine and 4 different processing time products of processed Radix Polygoni muhiflofi ; using high performance liquid chromatography method for determining the content of antilraquinone between raw medi- cine and processed products of in Radix Polygoni multiflori, revealing the relationship of quantity-time-effect between different samples with different processing time and diarrhoea causative intensity , and establishing the mathematical model of purgative effect of Polygonum muhiflorum Thunb. Results ： The required EDS0 dosage of different processing time of Polygonum muhiflo- rum respectively： 2. 562, 4. 085, 11. 146, 14. 692, 21. 068g·kg^-1（ crude drug·weight-1 ） ; purgative intensity sequence was ： the raw〉l h〉2h〉3h〉4h ; combined anthraquinone content in order ： the raw〉 1〉2h〉3h〉4h. Conclusion： There are obvious differences of purgative strength between raw product and processed Radix Polygoni Muitiflori , it has positive relationship between purgative strength and combined anthraquinone content on quantity-time-effect; associating biological detection method with mathematical model, the quality of traditional Chinese medicine and clinical medication safety will more comprehensively guaranteed.