| RP-HPLC法测定维生素C粉针剂中主药和有关物质的含量 |
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| 引用本文： | 王伟,刘永锁,陈振玲,刘国如.RP-HPLC法测定维生素C粉针剂中主药和有关物质的含量[J].亚太传统医药,2011,7(9):37-38. |
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| 作者姓名： | 王伟 刘永锁 陈振玲 刘国如 |
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| 作者单位： | 中国民用航空局民用航空医学中心,北京,100123 |
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| 摘 要： | 目的:建立RP-HPLC法测定维生素C粉针剂中维生素C和有关物质的含量。方法:采用的色谱柱为Phenomenex luna氨基柱(5μm,4.6mm×250mm),柱温25℃,流动相为0.02mol.L-1的磷酸二氢钾液(pH=3.0)和乙腈的混合液(体积比1∶1),系统流速1.0mL.min-1,检测波长为245nm。结果:该方法的线性范围为5.0~30.0μg.mL-1,标准曲线的相关系数为0.999 5;日内和日间精密度的RSD分别小于0.53%、2.98%;平均加样回收率(n=9)为99.12%,RSD=2.44%。结论:此法灵敏度高,结果准确,重现性好,可有效控制维生素C粉针剂的质量。
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| 关 键 词： | 维生素C 高效液相色谱法 含量测定 |
Determination of Main Component and Related Substances in Vitamin C Powder Injection by RP-HPLC |
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| Wang Wei,Liu Yongsuo,Chen Zhenling,Liu Guoru.Determination of Main Component and Related Substances in Vitamin C Powder Injection by RP-HPLC[J].Asia-Pacific Traditional Medicine,2011,7(9):37-38. |
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| Authors: | Wang Wei Liu Yongsuo Chen Zhenling Liu Guoru |
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| Institution: | Wang Wei,Liu Yongsuo,Chen Zhenling,Liu Guoru(Civil Aviation Medicine Center,Civil Aviation Administration of China,Beijing 100123,China) |
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| Abstract: | Objective：To develop the RP-HPLC method for the quantitative analysis of main component and related substances in vitamin C powder injection.Methods：The liquid chromatography was carried out using a NH2 column（5μm,4.6×250mm） with detection wavelength at 245nm.The mobile phase as follows：acetonitrile-dibasic potassium phosphate buffer solution（0.02mol.L-1,pH=3.0）=1∶1.The flow rate was 1mL·min-1,and column temperature was at 25℃.Results：The calibration curve was linear in the range of 5.0~30.0μg·mL-1,r=0.999 5.Average recovery（n=9） was 99.12%,RSD=2.44%.RSD of inter-day and within-day precision was less than 0.53% and 2.98% respectively.Conclusion：This method was found to be sensitive,and accurate for the quality control of vitamin C powder injection. |
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| Keywords: | Vitamin C Related substances RP-HPLC Determination |
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