球囊损伤血管内皮后平滑肌细胞凋亡相关基因表达及粉防已碱的作用

目的 :研究球囊损伤后血管平滑肌细胞 （VSMC）凋亡机制及粉防已碱 （Tet）对 VSMC凋亡及凋亡相关基因表达的影响。方法 :采用末端脱氧核苷酸转移酶介导的三磷酸脱氧尿嘧啶缺口末端标记法 （TU NEL ）和免疫组化技术检测球囊损伤内皮后 VSMC凋亡及 bcl- 1,bax蛋白表达的变化。结果 :球囊损伤后血管壁增生 ,血管中膜和内膜出现细胞凋亡。 Tet可明显促进 VSMS凋亡、降低球囊损伤后内膜及中膜厚度、增加管腔面积 （P<0 .0 5 ） ,促进 bax蛋白的表达、抑制 bcl- 2蛋白的表达 （P<0 .0 5 ）。结论 :bax,bcl- 2参与了球囊损伤内皮后血管平滑肌细胞凋亡的调节 ,Tet增加 VSMC细胞凋亡 ,防止血管再狭窄

Effect of tetrandrine on expression of apoptosis related-gene of vascular smooth muscle cells after balloon injury to vessel

AIM:to investigate the mechanism of vascular smooth muscle cells (VSMC) apoptosis after balloon injury. To study the effect of Tet on the apoptosis and apoptosis related-gene expression of VSMC. METHODS:Apoptosis of VSMC,Bax protein and Bcl-2 protein was measured by TUNEL and immunohistochemical technique after balloon injury. RESULTS:After balloon injury,the vascular was proliferated. Apoptosis of VSMC appeared in intima and media of the vascular. Tet significantly increase the apoptosis of VSMC and decrease the thickness of intima an media after ballon injury (*"P*"<0.05),and also can increase the area of vascular luminal. Tet significantly increase the expression of Bax protein and decrease the expression Bcl-2 protein(*"P*"<0.05). CONCLUSION:Bax and bcl-2 contribute to the regulation of VSMC apoptosis after balloon injury. Tet can increase the apoptosis of VSMc by the regulation of Bax and Bcl-2 gene. The Tet could significantly inhibit VSMC proliferation and prevent the restenosis of blood vessel after balloon injury.