| 雄激素及其受体对maspin启动子的抑制作用 |
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| 引用本文： | 贺美兰,张建业,张莲英,姜安丽,于雪艳,张鹏举.雄激素及其受体对maspin启动子的抑制作用[J].山东大学学报(医学版),2004,42(1):9-11,15. |
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| 作者姓名： | 贺美兰 张建业 张莲英 姜安丽 于雪艳 张鹏举 |
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| 作者单位： | 山东大学医学院生物化学与分子生物学研究所,山东,济南,25000O |
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| 基金项目： | 国家自然科学基金资助项目(30171026) |
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| 摘 要： | 目的：检测雄激素及其受体对maspin基因启动子的作用。方法：构建含maspin基因5侧启动子区846(-759， 87)bpDNA的荧光素酶报告基因表达载体pGL3-mP，然后与雄激素受体表达载体(hAR)共转染雄激素非依赖型前列腺癌细胞株PC-3M，用或不用雄激素处理细胞，48h后应用双荧光素酶测定系统，检测荧光素酶的表达活性。结果：hAR可抑制maspin启动子的表达活性，其抑制作用是雄激素配体非依赖性的。结论：maspin启动子区可能含有与雄激素受体结合的负性调控元件。
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| 关 键 词： | 受体 雄激素 雄激素类 启动区 基因 报告 |
| 文章编号： | 1671-7554(2004)01-0009-03 |
The suppression of androgen and its receptor on the promoter of maspin |
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| HE Mei-lan,ZHANG Jian-ye,ZHANG Lian-ying,et al.The suppression of androgen and its receptor on the promoter of maspin[J].Journal of Shandong University:Health Sciences,2004,42(1):9-11,15. |
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| Authors: | HE Mei-lan ZHANG Jian-ye ZHANG Lian-ying |
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| Abstract: | Objective: To detect the effect of androgen and its receptor on the promoter of maspin. Methods: pGL3-mP luciferase expression vector containing 846bp(-759, 87) DNA of maspin gene 5'promoter region was constructed. Then pGL3-mP plasmid and androgen receptor expression vecetor were cotransfected into PC3-M cell treated with or without androgen. Through detecting the activity of luciferase, the effect of androgen and its receptor on the activity of maspin promoter was studied. Results: Androgen receptor suppressed the activity of maspin promoter and its effect was independent from androgen. Conclusion: It is possible that the region of maspin promoter contains negative regulatory elements combined with androgen receptor. |
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| Keywords: | Receptors androgen Androgens Promoter regions Genes report |
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