| 杭州地区2014-2015年急性胃肠炎患者感染诺如病毒的流行病学特征分析 |
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| 引用本文： | 崔大伟,李中杰,林洁,靳淼,杨先知,谢国良,郑书发,余斐,陈瑜.杭州地区2014-2015年急性胃肠炎患者感染诺如病毒的流行病学特征分析[J].中华流行病学杂志,2016,37(2):254-258. |
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| 作者姓名： | 崔大伟 李中杰 林洁 靳淼 杨先知 谢国良 郑书发 余斐 陈瑜 |
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| 作者单位： | 310003 杭州, 浙江大学医学院第一附属医院检验科;310003 杭州, 浙江大学医学院第一附属医院检验科;310003 杭州, 浙江大学医学院第一附属医院检验科;310003 杭州, 浙江大学医学院第一附属医院检验科;310003 杭州, 浙江大学医学院第一附属医院检验科;310003 杭州, 浙江大学医学院第一附属医院检验科;102206 北京, 中国疾病预防控制中心传染病预防控制处, 传染病监测预警中国疾病预防控制中心重点实验室;310002 杭州市清波街道社区卫生服务中心检验科;102206 北京, 中国疾病预防控制中心病毒病预防控制所 |
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| 基金项目： | 国家科技重大专项(2012ZX10004-210) |
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| 摘 要： | 目的 了解2014-2015年杭州地区急性胃肠炎患者中诺如病毒的流行病学特征。方法 收集1 109份急性胃肠炎患者的临床流行病学资料和粪便样本,采用一步法双重荧光RT-PCR对样本中诺如病毒(GⅠ、GⅡ型)进行初筛,然后随机选择诺如病毒阳性的样本核酸进行多重RT-PCR扩增,对产物进行测序分析。结果 1 109份的粪便样本中,诺如病毒检出率为26.87%(298/1 109),以GⅡ亚型为主,占25.52%(283/1 109),GⅠ亚型占1.35%(15/1 109);不同性别人群诺如病毒检出率差异无统计学意义(P>0.05);不同年龄组人群中诺如病毒检出率分别为16.94%(<5岁)、19.45%(5~18岁)和32.26%(≥18岁);全年均可检出诺如病毒感染,高发季节为12月至次年3月,阳性率为29.67%~37.08%。序列分析显示,GⅡ基因亚型中以GⅡ-4/Sydney株为主(46.43%,13/28),其次为GⅡ-4/2006b株;GⅠ基因亚型中以GⅠ-1亚型为主(55.56%,5/9)。结论 诺如病毒是导致2014-2015年杭州地区急性胃肠炎的主要病原之一,GⅡ基因亚型特别是GⅡ-4/Sydney变异株和GⅡ-4/2006b变异株是该地区流行的优势毒株。
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| 关 键 词： | 诺如病毒 急性胃肠炎 聚合酶链反应 基因型 |
| 收稿时间： | 2015/8/17 0:00:00 |
Epidemiologic characteristics of noroviruses isolated in outpatients with acute gastroenteritis in Hangzhou area, from 2014 to 2015 |
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| Cui Dawei,Li Zhongjie,Lin Jie,Jin Miao,Yang Xianzhi,Xie Guoliang,Zheng Shuf,Yu Fei and Chen Yu.Epidemiologic characteristics of noroviruses isolated in outpatients with acute gastroenteritis in Hangzhou area, from 2014 to 2015[J].Chinese Journal of Epidemiology,2016,37(2):254-258. |
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| Authors: | Cui Dawei Li Zhongjie Lin Jie Jin Miao Yang Xianzhi Xie Guoliang Zheng Shuf Yu Fei and Chen Yu |
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| Institution: | Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;Department of Clinical Laboratory, the Fist Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;Division of Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, State Key Laboratory for Communicable Disease Surveillance and Early Warning, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Department of Clinical Laboratory, Center of Community Health Service of Qingbo Street, Hangzhou 310002, China;National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | |
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| Keywords: | Noroviruses Acute gastroenteritis Polymerase chain reaction Genotype |
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