D-半乳糖衰老模型大鼠学习记忆能力和特定脑区抗氧化酶活性的变化

目的 探讨D-半乳糖衰老模型大鼠学习记忆能力和特定脑区抗氧化酶活性的变化,以揭示学习记忆能力与特定脑区抗氧化酶活性的关系.方法 Wistar大鼠皮下注射D-半乳糖建立衰老模型,在MG-2型三等分辐射式迷宫中进行学习记忆能力的检测后,将大鼠立即断头处死,分别测定大脑皮层、小脑、纹状体、海马、下丘脑五个脑区SOD、CAT、GSH-Px活性.结果 D-半乳糖衰老模型大鼠迷宫正确反应率为(58.9±5.4)%,比对照组的(66.8±8.9)%明显降低(P＜0.05).24h后达标次数显著高于对照组衰老组(29.5±12.8)次,对照组(16.6±6.2)次,P＜0.01],24h正确反应率明显降低于对照组衰老组(67.3±10.3)%,对照组(79.1±6.9)%,P＜0.01].与对照组比较:皮层、海马、纹状体SOD活性(衰老组:66.12±5.89、80.46±6.57、87.82±6.87;对照组:57.63±4.21、67.46±6.80、68.37±6.52)显著降低(P＜0.05和P＜0.01);CAT活性在皮层、海马、纹状体、下丘脑四个脑区(衰老组:6.45±0.55、5.86±0.35、6.93±0.63、8.89±0.38;对照组:5.32±0.56、4.76±0.38、4.37±0.43、6.11±0.37)均显著降低(P＜0.05和P＜0.01);各脑区GSH-Px活性差异无显著性(P＞0.05).结论 D-半乳糖衰老模型大鼠学习记忆能力降低.衰老过程中海马、皮层、纹状体、下丘脑的抗氧化酶活性变化与学习记忆密切相关.

The study on learning and memory ability and specific brain area antioxidant enzyme activities in D-galactose model rats

Objective To explore the relationship between learning and memory ability and antioxidant enzyme (SOD, CAT, GSH-Px)activities in specific brain areas of D-galactose model rats. Methods The aging models were induced by D-galactose in 3-month old rats, and the learning and memory ability of aging model was detected in model MG-2 maze. The activity of SOD, CAT and GSH-Px in brain cortex, cerebellum,hippocampus, striatum and hypothalamus were detected immediately after rats were executed. Results The learning and memory ability of D-ga-lactose aging model was lower than that of control group. The difference of antioxidant enzyme activity among different rat brain areas was significant. The correct response rate of D-galactose aging model was( 58.9 ± 5.4 ) %, which is significantly lower than that of the control group (66.8 ±8.9) %, P ＜ 0.05. The times of response required reaching the standard after 24h of aging model rats was significantly higher than that of the control( Aging group :29.5 ± 12.8, Control group 16.6 ± 6.2; P＜ 0.01 ). Correct response rate after 24h was significantly lower than that of the control ( Aging group :67.3 ± 10.3 , Control group: 79.1 ± 6.9; P＜ 0.01 ). The SOD activity in cortex, hippocampus and striatum( Aging group :66.12 ±5.89,80.46 ± 6.57,87.82 ± 6.87; Control group :57.63 ± 4.21,67.46 ± 6.80,68.37 ± 6.52) significantly reduced ( P ＜ 0.05 and P ＜ 0.01 ).The CAT activity in cortex, hippocampus, striatum and hypothalamus( Aging group :6.45 ± 0.55,5.86 ±0.35,6.93 ± 0.63,8.89 ± 0.38; Control group :5.32 ± 0.56,4.76 ± 0.38,4.37 ± 043,6.11 ± 0.37) significantly reduced ( P ＜ 0.05 and P ＜ 0.01 ). GSH-Px activity was similar in each brain area without significance ( P ＞ 0.05 ). Conclusions The learning and memory ability of aging mode rats decreased. The change of antioxidant enzyme activities in hippocampus, cortex, striatum and hypothalamus is closely related to learning and memory ability.