| 辅助性T细胞17及IL-17在支气管哮喘小鼠中的变化 |
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| 引用本文： | 周 辰,殷凯生,陈军浩,史 莹.辅助性T细胞17及IL-17在支气管哮喘小鼠中的变化[J].南京医科大学学报,2009,29(8):1095. |
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| 作者姓名： | 周 辰 殷凯生 陈军浩 史 莹 |
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| 作者单位： | 周辰,殷凯生,史莹,ZHOU Chen,YIN Kai-sheng,SHI Ying(南京医科大学第一附属医院呼吸科,江苏,南京,210029);陈军浩,CHEN Jun-hao(南京大学医学院附属鼓楼医院检验中心,江苏,南京,210008)
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| 摘 要： | 目的:探讨辅助性T细胞17(Th17)及IL-17在支气管哮喘小鼠中的变化及意义.方法:将24只BABL/c小鼠随机分为哮喘组(12只)和正常对照组(12只).用卵清白蛋白(OVA)致敏和激发建立小鼠哮喘模型;行苏木精书红(HE)染色,观察肺组织炎症改变;流式细胞术检测脾脏单个核细胞中Th17细胞比例;酶联免疫吸附法(ELISA)检测支气管肺泡灌洗液(BALF)中卟17细胞相关细胞因子I-17水平;并计数BALF中细胞总数、嗜酸粒细胞及中性粒细胞数.结果:哮喘组肺组织及气道周围的炎症改变较正常对照组显著增强.哮喘组脾脏中Th17细胞及BALF中IL-17水平均显著高于正常对照组(P均<0.01).哮喘组BALF中细胞总数及嗜酸粒细胞计数显著高于正常对照组(P均<0.01),结论:哮喘小鼠存在Th17细胞数量增多及其分泌的IL-17水平增高,IL-17可能通过促进嗜酸粒细胞在气道的聚集而参与炎症反应,Th17细胞可能在哮喘的发病中具有重要作用.
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| 关 键 词： | 哮喘 Th17细胞 |
| 收稿时间： | 2009/3/20 0:00:00 |
Changes of the ratio of T helper 17 cells and the level of IL-17 in bronchial asthmatic mice |
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| ZHOU Chen,YIN Kai-sheng,CHEN Jun-hao and SHI Ying.Changes of the ratio of T helper 17 cells and the level of IL-17 in bronchial asthmatic mice[J].Acta Universitatis Medicinalis Nanjing,2009,29(8):1095. |
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| Authors: | ZHOU Chen YIN Kai-sheng CHEN Jun-hao and SHI Ying |
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| Institution: | Department of Respiratory Medicine;the First Affiliated Hospital of NJMU;Nanjing 210029;1Clinical Test Center;Drum Tower Hospital Affiliated to Medical School of Nanjing University;Nanjing 210008;China |
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| Abstract: | Objective:To investigate the changes of T helper 17 cells and the level of IL-17 in bronchial asthmatic mice. Methods: Twenty-four BABL/c mice were randomly divided into two groups,asthma group and control group. The asthmatic mouse models were established after ovalbumin(OVA) sensitization and challenge. The airway inflammation of lung tissue was observed by hematoxylin and eosin (HE) staining. The ratio of Th17 cells to mononuclear cells in the spleen was detected by flow cytometry. The level of IL-17 in ... |
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| Keywords: | IL-17 |
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