| 螺旋藻对阿霉素肾硬化大鼠肾脏的保护作用 |
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| 引用本文： | 孙,晓,王兆华,王,蕾.螺旋藻对阿霉素肾硬化大鼠肾脏的保护作用[J].泰山医学院学报,2014(3):168-170. |
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| 作者姓名： | 孙 晓 王兆华 王 蕾 |
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| 作者单位： | 泰山医学院附属泰山医院,山东泰安271000 |
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| 基金项目： | 泰安市科技局科研计划项目（NO.2008） |
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| 摘 要： | 目的研究螺旋藻对阿霉素肾硬化大鼠肾脏的保护作用及相关机制。方法将32只SD大鼠随机分为4组：假手术组（S组）,阿霉素肾硬化对照组（M组）,螺旋藻组（SP组）,贝那普利治疗组（B组）。8周后观察各组大鼠24 h尿蛋白定量、血尿素氮（BUN）、肌酐（Scr）及肾脏病理改变,免疫组化观察肾脏组织纤连蛋白（FN）、IV型胶原（Col IV）、转化生长因子β1（TGF-β1）的表达。RT-PCR测定肾皮质TGF-β1mRNA的表达。结果螺旋藻能减少尿蛋白、降低BUN和Scr,减轻基质增生和肾小球硬化,且能减少FN、Col IV、TGF-β1的表达。下调肾皮质TGF-β1mRNA的表达。结论螺旋藻能减轻阿霉素肾硬化大鼠肾脏损害,对肾脏有保护作用。
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| 关 键 词： | 螺旋藻 肾小球硬化症 FN TGF-β mRNA |
Renoprotect effect of spirulina on adriamycin-induced glomerulosclerosis in rats |
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| SUN Xiao;WANG Zhao-hua;WANG Lei.Renoprotect effect of spirulina on adriamycin-induced glomerulosclerosis in rats[J].Journal of Taishan Medical College,2014(3):168-170. |
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| Authors: | SUN Xiao;WANG Zhao-hua;WANG Lei |
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| Institution: | SUN Xiao;WANG Zhao-hua;WANG Lei;Dept. of Nephrology,Taian Central Hospital; |
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| Abstract: | Objective： To investigate the effects and mechanism of spirlina on adriamycin-induced glomerulosclerosis in rats. Methods： Thirty-two Sprague Dawley rats were randomly divided into sham operation,glomerulosclerosis model,spirlina treatment and benazepril groups. The 24-hour urinary protein excretion,Blood urea nitrogen（ BUN） and creatinine were measured after 8 weeks. Renal pathology and immunohistochemistry were assayed to measure the expression of fibronectin（ FN）,collagenIV（ Col IV） and transformer growth factorβ1（ TGF-β1）. RT-PCR was used to examine the TGF-β1mRNA. Results： The proteinuria,BUN and serum creatinine declined significantly（ P 0. 01） and lowed the expressions of FN,Col IV,TGF-β1and TGF-β1mRNA（ P 0. 01）. Conclusion： Spirlina plays an important role in the prevention and treatment for glomerulosclerosis. |
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| Keywords: | spirlina glomerulosclerosis FN TGF-βmRNA |
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