姜黄素对高糖刺激下大鼠肾脏系膜细胞血红素氧化酶-1表达的影响

目的 观察高糖刺激下大鼠肾脏系膜细胞(mesangial cell,MC)血红素氧化酶-1(heme oxygenase-1,HO-1)的表达及姜黄素对HO-1表达的影响.方法 将MC分为6组,正常对照组给予正常培养液,高糖刺激各组给予含 30 mmol/L葡萄糖的培养液,分别于培养6、12、24、48 h后收集细胞,另1组给予含30 mmol/L葡萄糖和33.33 mmol/L姜黄素的培养液共孵育6 h后收集细胞.分别用逆转录多聚酶链式反应(RT-PCR)和Western blot分析法检测各组系膜细胞HO-1 mRNA和蛋白的表达情况.结果 正常MC有微量的HO-1 mRNA表达,高糖刺激后HO-1 mRNA表达量明显增加,于6 h达到高峰, HO-1蛋白表达的变化趋势与mRNA变化趋势相同.MC加高糖和姜黄素共孵育6 h后,HO-1表达较单纯高糖刺激6 h组显著增强,其mRNA吸光度相对值分别为(1.987±0.236)、(1.195±0.167)(P<0.01),HO-1蛋白吸光度值分别为(23 560±1 867)、(14 072±1 333)(P<0.01).结论 高糖刺激下,系膜细胞HO-1 mRNA和蛋白表达呈时间依赖性变化,姜黄素可诱导高糖刺激下系膜细胞HO-1表达增强,从而保护细胞.

Effects of Curcumin on the Expression of HO-1 in Mesangial Cells Treated with High Glucose

Objective To investigate the expression of HO-1 in mesangial cells(MC) treated with high glucose and explore the effects of curcumin on the expression of HO-1.Methods MC were randomly divided into 6 groups.The control was given normal culture medium.Four groups were given culture medium with 30 mmol/L glucose and collected after 6,12,24 and 48 h respectively.The remaining group was given culture medium supplemented with 30 mmol/L glucose and curcumin(33.33 mmol/L).The cells were collected 6 h later.Reverse ...